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Plan

plan

1. A draught or form; properly, a representation drawn on a plane, as a map or a chart; especially, a top view, as of a machine, or the representation or delineation of a horizontal section of anything, as of a building; a graphic representation; a diagram.

2. A scheme devised; a method of action or procedure expressed or described in language; a project; as, the plan of a constitution; the plan of an expedition. God's plans like lines pure and white unfold. (M. R. Smith)

3. A method; a way of procedure; a custom. The simple plan, That they should take who have the power, And they should keep who can. (Wordsworth) body plan, floor plan, etc. See Body, Floor, etc.

Synonym: scheme, draught, delineation, plot, sketch, project, design, contrivance, device. See Scheme.

Origin: F, fr. L. Planus flat, level. See Plain.


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Hi guys, I'm a new guy in molecular biology. I plan to express a scfv in S2 cell using the plasmid pMT/bip/V5-His A (with enzyme site BglII and XbaI). Because there's a Bip signal sequence ahead of the enzyme site BglII, so I CAN NOT add Kozak sequence ...

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by yiwi123
Tue Mar 12, 2013 8:04 pm
 
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Topic: How can I add a kozak sequence in plasmid pMT/bip/V5-His A
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Views: 35

Conditional knockout mouse

... mouse line with Tamoxifen inducible Cre line, CAGGCre-ERTM, I get mice with with heterozygous Cre and floxed gene of interest. The experimental plan was to isolate myoblasts from these mice and induce Cre in cell culture by addition of Tamoxifen. To my surprise, the cells isolated from these ...

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by aji01
Wed Feb 20, 2013 11:25 am
 
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Topic: Conditional knockout mouse
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Re: Protein isolation

Okay, after doing some reading, I have come up with a different plan for quantifying HIF-1 alpha, which involves running an ELISA after performing a nuclear extraction. However, all the protocols and kits I have seen recommend 1-10 million cells for a good ...

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by roth317
Tue Feb 19, 2013 7:28 pm
 
Forum: Molecular Biology
Topic: Protein isolation
Replies: 2
Views: 337

Protein isolation

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by roth317
Tue Feb 12, 2013 9:25 pm
 
Forum: Molecular Biology
Topic: Protein isolation
Replies: 2
Views: 337

Re: The general theory of origin of life

... of life has no value. God paints a picture of the material world via the events using assistants - mathematics and geometry. To implement the Plan, a technologies are important , which must build on the basic, fundamental things and designs. Most of the physical constants have been found, ...

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by leniel
Thu Feb 07, 2013 10:15 pm
 
Forum: General Discussion
Topic: The general theory of origin of life
Replies: 11
Views: 1170
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