Dictionary » P » Pipette

Pipette

pipette

A graduated tube (marked in ml) used to transport a definite volume of a gas or liquid in laboratory work.

Origin: Fr. Dim. Of pipe, pipe


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


peripheral mononuclear cells handling

Scraping them with a pipette might be bit difficult, but if you have to use 96-well plates then that might be the only option. Or do they have mini-scrapers for so small wells? You could try seeding the cells on a bigger plate and using ...

See entire post
by biohazard
Mon Nov 05, 2012 1:11 pm
 
Forum: Cell Biology
Topic: peripheral mononuclear cells handling
Replies: 3
Views: 2026

Re: peripheral mononuclear cells handling

Indeed want to study monocytes. I think I'm going to use PBS/edta and to scrap them with the tips of the pipette. Do you think it's a good idea?

See entire post
by claire84
Fri Nov 02, 2012 2:56 pm
 
Forum: Cell Biology
Topic: peripheral mononuclear cells handling
Replies: 3
Views: 2026

peripheral mononuclear cells handling

... most cell culture plates) and must be removed with PBS/EDTA & cell scraper, or perhaps with trypsin. For other PBMCs resuspending them with a pipette is sufficient. The tendency of monocytes to adhere to culture plates can also be easily used to reduce the contamination by monocytes if you ...

See entire post
by biohazard
Fri Nov 02, 2012 8:46 am
 
Forum: Cell Biology
Topic: peripheral mononuclear cells handling
Replies: 3
Views: 2026

Re: Exercises for better coordination/dexterity in lab?

I face the same problems at my workplace. Especially the pipette-tip-glassware-wall problem. It's one of those things that comes with practice and muscle memory, I think. I've become a lot more proficient now that I'm doing it hours a day, every day. ...

See entire post
by PsycoCarrot
Fri Jul 06, 2012 11:15 pm
 
Forum: Microbiology
Topic: Exercises for better coordination/dexterity in lab?
Replies: 2
Views: 2598

washing bacteria

... then add PBS and then spin again, pour off s/n add more PBS and spin... OR DOES IT MEAN just add PBS ensuring the pellet doesn't break up and then pipette out the PBS and then add more and take it out..? cheers, b_06er

See entire post
by biology_06er
Sun Jun 24, 2012 11:13 am
 
Forum: General Discussion
Topic: washing bacteria
Replies: 1
Views: 972
View all matching forum results

This page was last modified 21:16, 3 October 2005. This page has been accessed 4,554 times. 
What links here | Related changes | Permanent link