Pipette

Scraping them with a pipette might be bit difficult, but if you have to use 96-well plates then that might be the only option. Or do they have mini-scrapers for so small wells? You could try seeding the cells on a bigger plate and using ...

See entire post

Indeed want to study monocytes. I think I'm going to use PBS/edta and to scrap them with the tips of the pipette. Do you think it's a good idea?

See entire post

... most cell culture plates) and must be removed with PBS/EDTA & cell scraper, or perhaps with trypsin. For other PBMCs resuspending them with a pipette is sufficient. The tendency of monocytes to adhere to culture plates can also be easily used to reduce the contamination by monocytes if you ...

See entire post

I face the same problems at my workplace. Especially the pipette-tip-glassware-wall problem. It's one of those things that comes with practice and muscle memory, I think. I've become a lot more proficient now that I'm doing it hours a day, every day. ...

See entire post

... then add PBS and then spin again, pour off s/n add more PBS and spin... OR DOES IT MEAN just add PBS ensuring the pellet doesn't break up and then pipette out the PBS and then add more and take it out..? cheers, b_06er

See entire post