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Pierce

pierce

To enter; to penetrate; to make a way into or through something, as a pointed instrument does; used literally and figuratively. And pierced to the skin, but bit no more. (Spenser) She would not pierce further into his meaning. (Sir P. Sidney)


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protein extraction from fat breast tissue

... : normal bresat tissue is really fatty and the lipids interfere with my peptides quantification after trypsin digestion. I use the micro BCA from Pierce. The problem is that it seems to overestimate the amount of peptides in normal breast tissue lysates compared to tumor lysates, and I think this ...

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by Vero36
Sat May 04, 2013 1:18 pm
 
Forum: Cell Biology
Topic: protein extraction from fat breast tissue
Replies: 1
Views: 1808

doubt about Detoxi-Gelâ„¢ Endotoxin Removing Gel

... from the column about 90% of the bed volumn has been collected. this means after apply sample to column, there is 10% sample loss, isn't it? 2 The Pierce 1ml Detoxi-GEL column has a void volumn of 0.3-0.5ml. this means after apply sample to column, there is 0.3-0.5ml sample detained in column, ...

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by jasmina
Fri Jan 08, 2010 4:41 am
 
Forum: Cell Biology
Topic: doubt about Detoxi-Gelâ„¢ Endotoxin Removing Gel
Replies: 7
Views: 3718

B-per GST fusion protein purification

I need to purify GST tagged protein by using B-per reagent from pierce.(I used pGEX2T vector).Is there anyone used this kit? I have used Y-Per reagent before and I have loads of problems. My protein yield was so low. I had also low amount GST band except ...

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by bsengez
Mon Apr 13, 2009 8:36 pm
 
Forum: Molecular Biology
Topic: B-per GST fusion protein purification
Replies: 0
Views: 1172

Molecular biology of the gene

No, I read just 5th edition. I like B.Lewin. ''Genes.VIII'' and B. A. Pierce ''Genetics - A Conceptual Approach''.

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by MisterATP
Wed Jul 23, 2008 5:53 pm
 
Forum: Genetics
Topic: Molecular biology of the gene
Replies: 1
Views: 1833

how did you purchase reagents ?

... maybe the "agency" problem just happen in the developing countries. you know, we have to place an order to the companies such as Sigma, Pierce, ABcam,R&D. none of them is located in china. but I am little surprised with maryli. I thought Japan can provide much qualified products ...

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by enhancer chen
Tue Nov 27, 2007 1:46 am
 
Forum: Cell Biology
Topic: how did you purchase reagents ?
Replies: 5
Views: 1916
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