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Phosphodiester bond

phosphodiester bond

(Science: chemistry) Not a precise term. Refers to any molecule in which two parts are joined through a phosphate group.

Examples are found in rNA, dNA, phospholipids, cyclic nucleotides, nucleotide diphosphates and triphosphates.


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What happens to free phosphates after DNA elongation in PCR?

... the nucleotide are removed (creating the energy necessary to form the phosphodiester bond that attaches the remaining phosphate group to the growing chain). What happens to those ...

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by Melstmu2010
Thu Jun 23, 2011 8:43 pm
 
Forum: General Discussion
Topic: What happens to free phosphates after DNA elongation in PCR?
Replies: 1
Views: 1072

Re: DNA backbone breakage

... made up of alternative sugar and phosphate are held together by Phosphodiester bond. Phosphodiester bond is a covalend bond, so energy is required to more for breaking phosphodiester ...

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by kanagasundar
Tue Oct 05, 2010 3:54 am
 
Forum: Genetics
Topic: DNA backbone breakage
Replies: 5
Views: 5448

Re: DNA Replication Lagging Strand

... to where the primer was) DNA Ligase DNAse breaks up DNA at the phosphodiester bond, and RNA polymerase makes RNA. I still don't know which one of these isn't used.

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by firecloak
Fri Sep 17, 2010 7:03 pm
 
Forum: Genetics
Topic: DNA Replication Lagging Strand
Replies: 6
Views: 4506

Re: Generation of DNA nucleotides

... into the template nucleotide. If the nucleotide triphosphate hydrogen bonds correctly with the template nucleotide, the new phosphodiester linkage forms. If the nucleotide triphosphate is the wrong one then usually ...

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by jonmoulton
Thu Jan 14, 2010 5:11 pm
 
Forum: Molecular Biology
Topic: Generation of DNA nucleotides
Replies: 65
Views: 43275

Re:

... DNA, which newly formed end will retain the phosphate group from the phosphodiester bond? Is it the 3' or the 5'-end? See above ;) Regarding the Kozak' sequence, the note was ...

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by JackBean
Fri Oct 09, 2009 7:23 am
 
Forum: Molecular Biology
Topic: Gene cloning question
Replies: 18
Views: 13395
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