Dictionary » P » Phosphates

Phosphates

(noun) Plural form of phosphate.

See phosphate for definition and additional information.


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What makes dominant allele dominant?

... have change of a crucial residue to turn off your protein, like in case of Asp to Asn mutations in receptor which impair its ability to transfer phosphates and thus signal the binding. Or you could change the post-translational modification and thus mis-localizing the protein or impairing its ...

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by JackBean
Thu Feb 27, 2014 5:20 pm
 
Forum: Genetics
Topic: What makes dominant allele dominant?
Replies: 5
Views: 3692

MOLECULAR CLONING

Not to mention, that synthesized oligos have 5'-OH and 3'-phosphate ends... DNA ligase removes 3'-phosphates and replaces it with -OH. Thus, synthesized oligos without polynucleotide kinase treatment will participate in the ligation reaction with both their ends having -OH ...

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by kk
Fri Jul 19, 2013 12:50 pm
 
Forum: Molecular Biology
Topic: MOLECULAR CLONING
Replies: 9
Views: 5252

MOLECULAR CLONING

... a related question. I try to ligate annealed oligos into double digested (different enzymes) plasmid. The oligos I annealed were not modified with phosphates. Do I need to phosphorylate or dephosphorylate any of the two? As far as I understand, it is not necessary to dephosphorylate my double-digested ...

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by kk
Tue Feb 12, 2013 2:48 pm
 
Forum: Molecular Biology
Topic: MOLECULAR CLONING
Replies: 9
Views: 5252

Experiment on weathering

... 3 types of weathering. They are mechanical, chemical, and biological. It might be weathering on a rock or a crystal. Then I want to see how much phosphates come out. Is this experiment possible?

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by ArcaneIllumination
Sun Dec 09, 2012 5:25 pm
 
Forum: Ecology
Topic: Experiment on weathering
Replies: 1
Views: 1581

Re: Help - Why can't DNA be parallel?

... strand. I understand this, otherwise there is no way they could base pair. However, they have NOT been flipped UPSIDE DOWN, unlike the riboses/phosphates which have been. The thing I don't understand (ish, now), is why you can't have that ribose/phosphate backbone flipped right side up in the ...

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by keetner
Tue Oct 02, 2012 9:07 pm
 
Forum: Molecular Biology
Topic: Help - Why can't DNA be parallel?
Replies: 7
Views: 4968
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