Phosphate

... endergonic until ATP or GTP are utilized, effectively making the process exergonic. For example, the pathway leading from pyruvate to glucose-6-phosphate requires 4 molecules of ATP and 2 molecules of GTP. Gluconeogenesis is often associated with ketosis. Gluconeogenesis is also a target of ...

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... a related question. I try to ligate annealed oligos into double digested (different enzymes) plasmid. The oligos I annealed were not modified with phosphates. Do I need to phosphorylate or dephosphorylate any of the two? As far as I understand, it is not necessary to dephosphorylate my double-digested ...

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Although the Sodium Pump is an ATPase, during it's cycle there is a phosphate group being added and taken away from the protein so surely kinase and phosphotase are involved because an ATPase only hydrolyses ATP? Also, we are just going over how Enzymes work ...

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Hi folks, I'm reading about this coupled reaction between hexokinase/glucose-6-phosphate dehydrogenase in regards to spectrophotometry. The reaction I'm given is: glucose + ATP ----(hexokinase)---> G-6-P + ADP G-6-P + NADP+ <---(G-6-P dehydrogenase)---> ...

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... strand. I understand this, otherwise there is no way they could base pair. However, they have NOT been flipped UPSIDE DOWN, unlike the riboses/phosphates which have been. The thing I don't understand (ish, now), is why you can't have that ribose/phosphate backbone flipped right side up in the ...

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