Dictionary » P » Phosphate

Phosphate

Definition

noun, plural: phosphates

A salt of phosphoric acid.


Supplement

As a biological molecule, it is composed of phosphorus and oxygen and plays a major role in biological processes of many organisms, e.g. as chemical component of nucleic acids (DNA and RNA), nucleotides (ATP), plasma membrane (phospholipids), etc.

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Gluconeogenesis

... endergonic until ATP or GTP are utilized, effectively making the process exergonic. For example, the pathway leading from pyruvate to glucose-6-phosphate requires 4 molecules of ATP and 2 molecules of GTP. Gluconeogenesis is often associated with ketosis. Gluconeogenesis is also a target of ...

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by snowfall
Thu May 30, 2013 5:24 pm
 
Forum: Cell Biology
Topic: Gluconeogenesis
Replies: 7
Views: 1831

MOLECULAR CLONING

... a related question. I try to ligate annealed oligos into double digested (different enzymes) plasmid. The oligos I annealed were not modified with phosphates. Do I need to phosphorylate or dephosphorylate any of the two? As far as I understand, it is not necessary to dephosphorylate my double-digested ...

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by kk
Tue Feb 12, 2013 2:48 pm
 
Forum: Molecular Biology
Topic: MOLECULAR CLONING
Replies: 7
Views: 2242

Questions on the Sodium Potassium Pump and Enzymes

Although the Sodium Pump is an ATPase, during it's cycle there is a phosphate group being added and taken away from the protein so surely kinase and phosphotase are involved because an ATPase only hydrolyses ATP? Also, we are just going over how Enzymes work ...

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by JoWillyTso
Mon Nov 05, 2012 5:50 pm
 
Forum: Cell Biology
Topic: Questions on the Sodium Potassium Pump and Enzymes
Replies: 3
Views: 1545

Spectrophotometry - why can't some products be read?

Hi folks, I'm reading about this coupled reaction between hexokinase/glucose-6-phosphate dehydrogenase in regards to spectrophotometry. The reaction I'm given is: glucose + ATP ----(hexokinase)---> G-6-P + ADP G-6-P + NADP+ <---(G-6-P dehydrogenase)---> ...

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by keetner
Thu Nov 01, 2012 5:16 am
 
Forum: Molecular Biology
Topic: Spectrophotometry - why can't some products be read?
Replies: 2
Views: 1121

Re: Help - Why can't DNA be parallel?

... strand. I understand this, otherwise there is no way they could base pair. However, they have NOT been flipped UPSIDE DOWN, unlike the riboses/phosphates which have been. The thing I don't understand (ish, now), is why you can't have that ribose/phosphate backbone flipped right side up in the ...

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by keetner
Tue Oct 02, 2012 9:07 pm
 
Forum: Molecular Biology
Topic: Help - Why can't DNA be parallel?
Replies: 7
Views: 2142
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