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Phosphatase

phosphatase

(Science: enzyme) That hydrolyse phosphomonoesters. Acid phosphatases are specific for the single charged phosphate group and alkaline phosphatases for the double charged group. These specificities do not overlap.

The phosphatases comprise a very wide range of enzymes including broad and narrow specificity members. Phosphoprotein phosphatases specifically de phosphorylate a particular protein and are essential if phosphorylation is to be used as a reversible control system. Any of a group of enzymes that act as a catalyst in the hydrolysis of organic phosphates.A type of [[enzyme capable of releasing a [[phosphate from its parent molecule, e.g. the release of phosphate from [[ATP to release energy and the phosphate.


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Questions on the Sodium Potassium Pump and Enzymes

... sites, try to find out something about proof-reading mechanism. I don't know about the ATPase, but I don't remember any mention of kinase or phosphatase.

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by JackBean
Tue Nov 06, 2012 9:23 pm
 
Forum: Cell Biology
Topic: Questions on the Sodium Potassium Pump and Enzymes
Replies: 3
Views: 3793

ligation problem although try everything,suggestions?

... (NEB)alternatively try 25 minutes and also tried T4 DNA ligase at room temperature (NEB). I make 1:1, 1:3, 1:5 and 1:10 ligations. I make alkaline phosphatase and then try ligation again. I used 50 ng vector,15 ng insert it did not worked. I used 100 ng vector, it also not worked. I used invitrogen ...

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by genetherapy
Wed May 16, 2012 3:31 pm
 
Forum: Molecular Biology
Topic: ligation problem although try everything,suggestions?
Replies: 7
Views: 4329

Inactivate an enzyme

The number of ligated vectors should be lower than if you inactivated the phosphatase. Let us know, if you've got something positive.

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by JackBean
Fri Apr 13, 2012 8:06 am
 
Forum: Molecular Biology
Topic: Inactivate an enzyme
Replies: 7
Views: 2037

Inactivate an enzyme

Ok i understand but actually I had inactivated phosphatase in 65°C after ligation and it's correct i think, now i'm waiting for the results !

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by foufoulette
Thu Apr 12, 2012 12:01 am
 
Forum: Molecular Biology
Topic: Inactivate an enzyme
Replies: 7
Views: 2037

Inactivate an enzyme

What do you think? Will the phosphatase do something if you have there plenty of ATP and not-dephosphorylased insert DNA?

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by JackBean
Wed Apr 11, 2012 9:18 am
 
Forum: Molecular Biology
Topic: Inactivate an enzyme
Replies: 7
Views: 2037
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