A colorless to pale yellow reagent used to react with the amine group of the N-terminal amino acid, as used in Edman degradation


Edman degradation is a method used in sequencing amino acids in a peptide that consists of no more than 50 residues.

The phenylisothiocyanate is just one of the many reagents to label terminal amino acids. It reacts with the free N-terminal amino group of a peptide chain to ultimately form a phenylthiohydantoin relevant in the identification of N-terminal amino acids.

Molecular formula: C6H5NCS

Abbreviation: PITC



See also:

Mentioned in:

Please contribute to this project, if you have more information about this term feel free to edit this page

This page was last modified on 3 March 2011, at 09:37. This page has been accessed 5,603 times. 
What links here | Related changes | Permanent link