Edman degradation is a method used in sequencing amino acids in a peptide that consists of no more than 50 residues. It is just one of the many reagents used to label terminal amino acids. It reacts with the free N-terminal amino group of a peptide chain to ultimately form a phenylthiohydantoin relevant in the identification of N-terminal amino acids.
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... A pure protein was subjected to N-terminal analysis using c14-phenyl isothiocyanate,specific activity 120ci/mole.Reaction of the reagent with 2.3microgram of protein ...
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