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Phenyl isothiocyanate



The reagent used in Edman degradation to react with the amine group of the N-terminal amino acid


Edman degradation is a method used in sequencing amino acids in a peptide that consists of no more than 50 residues. It is just one of the many reagents used to label terminal amino acids. It reacts with the free N-terminal amino group of a peptide chain to ultimately form a phenylthiohydantoin relevant in the identification of N-terminal amino acids.

Molecular formula: C6H5NCS

Abbreviation: PITC



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Molecular weight of protein

... A pure protein was subjected to N-terminal analysis using c14-phenyl isothiocyanate,specific activity 120ci/mole.Reaction of the reagent with 2.3microgram of protein ...

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by khushi
Fri Oct 24, 2008 3:04 pm
Forum: Molecular Biology
Topic: Molecular weight of protein
Replies: 1
Views: 2105

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