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Phase separation

phase separation

(Science: chemistry) The separation of fluid phases that contain different concentrations of common components.

Occurs with partially miscible solvents used in many biochemical separation methods. Also temperature dependent phase separation occurs with some detergent solutions. With reference to membranes means the segregation of lipid components into domains that have different chemical composition.

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Basic Knowledge about DNA Extraction

... and centrifuged, the proteins will remain in the organic phase and can be drawn off and the DNA will be found at the interface between ... DNA. A centrifuge can spin at up to 15,000 rpm to facilitate separation of the different phases of the extraction and is used after the ...

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by Katy_Bobbles
Thu Jul 10, 2008 2:11 pm
Forum: Molecular Biology
Topic: totalRNA isolation for miRNA
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Topic: totalRNA isolation for miRNA
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Consider the separation principles of “ordinary biochemical methods”. They almost always depend on exploiting ... basis of density and shape; crystallization separates on the basis of thermodynamic phase properties, and so on. Now consider a sample of DNA. Is there any chemical or physical ...

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by blcr11
Sun Oct 14, 2007 7:16 am
Forum: Molecular Biology
Topic: Purifying DNA
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