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Phase plate

phase plate

(Science: microscopy) The plate used near the back focal plane of a microscope objective lens (in conjunction with an annulus at the front focal plane of the condenser lens) to achieve phase contrast. The phase plate selectively shifts the phase of the waves diffracted by the specimen by a quarter wave and reduces the amplitude of the undeviated, direct beam.

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The process of mitosis is divided into 6 stages. The Interphase, Prophase, Metaphase, Anaphase, Telophase, and cytokinasis. At Interphase, ... of the chromosomes and align the the chromosomes at the equatorial plate. 4. Anaphase The spindle fibres shorten and the centromere splits, separated ...

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by HerrBiologie
Sun Oct 30, 2011 9:16 am
Forum: Cell Biology
Replies: 2
Views: 5182

Meiosis = extreme confusion. Please help!

... 1- the cell goes throught the cell cycle #don't forget that in the s-phase the chromosome duplicate 2-the 1st stage of miosis is the prophase ... -in methaphase I the duplicated homologues align at the methaphase plate 4-the 4th stage is anaphase I in this stage the homologues migrate ...

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by nebeyuasrat
Fri Jul 22, 2011 1:22 am
Forum: Cell Biology
Topic: Meiosis = extreme confusion. Please help!
Replies: 10
Views: 60057

Re: Unusual cell division- Identifying mutant protein and genes

... replicate their DNA, divide the nucleus and get ready for the final phase and divide into two separate daughter cells. So my thoughts were : ... (pinch) containing a contractile ring develops where the metaphase plate used to be, pinching off the separated nuclei.[17] In both animal and ...

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by dharavsolanki
Sat Feb 19, 2011 3:07 pm
Forum: Molecular Biology
Topic: Unusual cell division- Identifying mutant protein and genes
Replies: 4
Views: 5233

sporulation in Bacillus

... trying to count the sporulation efficiency in my B.pumilus mutants by phase contrast microscopy as well with counting on normal LB agar plate ( after heat shock at 80deg for 20min)..but the results in microscopy showing around ...

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by praveen127
Thu Oct 07, 2010 3:08 pm
Forum: Microbiology
Topic: sporulation in Bacillus
Replies: 1
Views: 1983

Question about chromosome replication before mitosis

... the answer lies in the much historical definition of a chromosome. In S phase, each chromosome makes a copy of itself, but the two copies (called ... level. Now, in metaphase the chromosomes line up at the metaphase plate. At precisely the same time they split into the two daughter chromosomes ...

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by MrMistery
Sat Mar 07, 2009 5:40 pm
Forum: Genetics
Topic: Question about chromosome replication before mitosis
Replies: 1
Views: 8827
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