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Phagemid

phagemid

(Science: molecular biology) A phage whose genome contains a plasmid that can be excised by co-infection of the host with a helper phage.

Useful as vectors for library production, as the library can be amplified and screened as phage, but the inserts of selected plaques can readily be prepared as plasmids without subcloning.

Example of a commercial phagemid: _Zap, from which pBluescript can be excised with helper phage.


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Question about phage library

... commercially or constructed in house. The construction of a phage display library is accomplished by inserting DNA fragments into phage or phagemid genomes which proteins are expressed on the phage coat. This creates a direct physical link between the DNA sequences and their encoding proteins. ...

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by poloke
Sat Jun 02, 2012 2:06 pm
 
Forum: Microbiology
Topic: Question about phage library
Replies: 3
Views: 2670

T7 Expression System

... I just have a question and I hope that it's okay to ask here. I currently have a simple expression system setup within E.Coli K12 strains, using a phagemid with Tet-R/Amp-R genes, and my gene of interest inserted downstream of the T7 promoter. I plan to introduce a heat-inducible plasmid containing ...

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by TjCooper
Sat Dec 31, 2011 5:46 pm
 
Forum: Molecular Biology
Topic: T7 Expression System
Replies: 5
Views: 3482

Fab library constructing-ligation/transformation problem

... constructing work. I did PCR and got light chain (LC) products. There is a LC stuffer in the vector so I digested and gel purified the digested phagemid vector and the LC stuffer as a control. I tried to ligate the digested LC or LC stuffer with the vector. To test, I transformed the ligation ...

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by FABphage
Tue Jan 19, 2010 9:54 pm
 
Forum: Molecular Biology
Topic: Fab library constructing-ligation/transformation problem
Replies: 0
Views: 1197

ssDNA made by M13K07

Hi, has anyone here done the experiments about made ssDNA by M13K07? I have done this experiment recently,but I couldnot get the ssDNA of my phagemid. Could anyone tell me the reasons? Many thanks!

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by houlinlin
Sun Jul 06, 2008 7:41 am
 
Forum: Molecular Biology
Topic: ssDNA made by M13K07
Replies: 0
Views: 911

Re: Plasmid question

pBCSK, a phagemid has the T7 promotor in the MCS. I am uploading its map here. Would this alter your response? pBC_SK_plus.jpg

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by tittu
Tue May 27, 2008 2:45 pm
 
Forum: Molecular Biology
Topic: Plasmid question
Replies: 3
Views: 2591


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