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Phagemid

phagemid

(Science: molecular biology) A phage whose genome contains a plasmid that can be excised by co-infection of the host with a helper phage.

Useful as vectors for library production, as the library can be amplified and screened as phage, but the inserts of selected plaques can readily be prepared as plasmids without subcloning.

Example of a commercial phagemid: _Zap, from which pBluescript can be excised with helper phage.


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ssDNA made by M13K07

Hi, has anyone here done the experiments about made ssDNA by M13K07? I have done this experiment recently,but I couldnot get the ssDNA of my phagemid. Could anyone tell me the reasons? Many thanks!

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by houlinlin
Sun Jul 06, 2008 7:41 am
 
Forum: Molecular Biology
Topic: ssDNA made by M13K07
Replies: 0
Views: 454

Re: Plasmid question

pBCSK, a phagemid has the T7 promotor in the MCS. I am uploading its map here. Would this alter your response? pBC_SK_plus.jpg

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by tittu
Tue May 27, 2008 2:45 pm
 
Forum: Molecular Biology
Topic: Plasmid question
Replies: 3
Views: 831


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