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PHA

Definition

noun

Abbreviation for phytohemagglutinin, a hemagglutinin derived from plants, especially beans.


Supplement

Phytohemagglutinins or PHA are lectins capable of causing agglutination of erythrocytes and leukocytes. They are extracted from common beans (Phaseolus vulgaris), white kidney beans, green beans, fava beans, etc. They have various physiological effects, one of which is causing mitogenic effects, especially on T lymphocytes.


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Proliferation assay

... but does not affect the peaks once the cells start to divide - except that their intensity is high in general. Rather, you might have too much PHA. It is a very powerful mitogen and chances are that it makes all the cells divide -> all cells have undergone equal amounts of cell divisions ...

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by biohazard
Wed Jun 13, 2012 7:17 am
 
Forum: Cell Biology
Topic: Proliferation assay
Replies: 7
Views: 10772

Re: Proliferation assay

... PBMCS with CFSE (5micromol for 2-10million cells) and then staining with CD3. I sort the cells and then stimulate them for proliferation with PHA(2microlitre). I also stain the cells with CD4 and CD8 on the day of analysis. I add PI jus before running the cells on the flow cytometer to discriminate ...

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by hemhaspri
Wed Jun 13, 2012 6:29 am
 
Forum: Cell Biology
Topic: Proliferation assay
Replies: 7
Views: 10772

Re:

... with the plasmid? Why is that so important? because it's my isolate from environment. I still in progress of identify it and characterize the PHA production of the bacteria. that's why I need the bacteria,not only the plasmid.

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by azarulez
Sun Nov 22, 2009 10:47 am
 
Forum: Microbiology
Topic: help me..how to cultivate bacteria from lyophilized cell
Replies: 13
Views: 6030

Re: Proliferation assay

... I tried with some MM6 macrophages and K562 (to check if the cell type might make a difference). I am stimulating my T-cells with PMA instead of PHA, but as these twomitogens are pretty similar, I don't think this make a huge difference. I am counting my cells on a Neubauer chamber with trypan ...

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by spillou
Mon Jun 23, 2008 2:24 pm
 
Forum: Cell Biology
Topic: Proliferation assay
Replies: 7
Views: 10772

Proliferation assay

... a chamber, or just look at the colour of your medium? What cells do you use? For example T cells need proper stimulants to make them divide (e.g. PHA if you want unspecific proliferation), as well as the feeder cells. It is easy to think your cells proliferate, when the actual metabolism and medium ...

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by biohazard
Tue Jun 17, 2008 7:26 am
 
Forum: Cell Biology
Topic: Proliferation assay
Replies: 7
Views: 10772
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