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Dictionary » P » Pfu dna polymerase Pfu dna polymerasePfu DNA polymerase (Science: enzyme) From pyrococcus furiosus; shows sequence homology with alpha-like dNA polymerases; amino acid sequence given in first source Registry number: EC 2.7.7.- Synonym: pfu polymerase ![]()
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Results from our forumRe: PCR & Agarose gel electrophoresis... anneal? why are there two bands? did you use more than 2 primers? which polymerase did you use (proofreading or not)? does a primer anneal to the ... involving a contamination would be if the extraction of the cellular DNA went wrong. Do you have the gene already in a plasmid anywhere in your ...
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influence of temperature on biomolecules... also by shape/bi-stability) 4) transcription rate and errors (TF to DNA binding rate, polymerase binding rate, chromatin effects) 5) translation rate and errors Thanks a lot
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Cellular processes affected by temperature..... also by shape/bi-stability) 4) transcription rate and errors (TF to DNA binding rate, polymerase binding rate, chromatin effects) 5) translation rate and errors
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Paramutation... silencing requires the mop1 gene, which encodes an RNA-dependent RNA polymerase." This polymerase is required to maintain a threshold level ... other epigenetic changes, it involves a covalent modification of the DNA and/or the DNA-bound histone proteins without changing the DNA sequence ...
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Re: Ligation and expression trouble... is mainly that bases can be lost at the ends, or that it can ligate and produce a molecule that is missing a few bases, which get filled in by the polymerase of the bacterium after you transform your ligation mix. Oftentimes i'll get a whole bunch of re-ligated vector when I pick my colonies and ...
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