Pfu dna polymerase

... anneal? why are there two bands? did you use more than 2 primers? which polymerase did you use (proofreading or not)? does a primer anneal to the ... involving a contamination would be if the extraction of the cellular DNA went wrong. Do you have the gene already in a plasmid anywhere in your ...

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... also by shape/bi-stability) 4) transcription rate and errors (TF to DNA binding rate, polymerase binding rate, chromatin effects) 5) translation rate and errors Thanks a lot

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... also by shape/bi-stability) 4) transcription rate and errors (TF to DNA binding rate, polymerase binding rate, chromatin effects) 5) translation rate and errors

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... silencing requires the mop1 gene, which encodes an RNA-dependent RNA polymerase." This polymerase is required to maintain a threshold level ... other epigenetic changes, it involves a covalent modification of the DNA and/or the DNA-bound histone proteins without changing the DNA sequence ...

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... is mainly that bases can be lost at the ends, or that it can ligate and produce a molecule that is missing a few bases, which get filled in by the polymerase of the bacterium after you transform your ligation mix. Oftentimes i'll get a whole bunch of re-ligated vector when I pick my colonies and ...

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