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Peroxidase

peroxidase

(Science: enzyme) A haem enzyme that catalyses reduction of hydrogen peroxide by a substrate that loses two hydrogen atoms. Within cells, may be localised in peroxisomes. Coloured reaction products allow detection of the enzyme with high sensitivity, so peroxidase coupled antibodies are widely used in microscopy and eLISA. Lactoperoxidase is used in the catalytic surface labelling of cells by radioactive iodine.


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The effect of ethanol on peroxidase activity

... wondering if anyone knew why by increasing ethanol concentration in a 10mL solution mixed with yeast, a greater amount of oxygen was produced? So peroxidase activity increased when i increased ethanol concentration in my lab. I thought it was suppose to be the opposite since alcohol denatures ...

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by wayne2824
Fri Feb 14, 2014 10:44 pm
 
Forum: General Discussion
Topic: The effect of ethanol on peroxidase activity
Replies: 1
Views: 214

Re: Difference between ICC and IF

... for example: immmunihistochemistry using goat anti-VEGFR primary antibody and anti-goat-IgG secondary antibody conjugated to horseradish peroxidase.

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by jonmoulton
Mon Feb 25, 2013 5:11 pm
 
Forum: Molecular Biology
Topic: Difference between ICC and IF
Replies: 7
Views: 14371

Two-color in situ help

... embryos and the auto-fluorescence was so weak that it wouldn't even be picked up by our most sensitive cameras. I also suspected the endogenous peroxidase activity but that did not seem to factor in either since the anti-DIG + cy3 always worked. I thought of trying cy5 but we do not seem to ...

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by vastgenome
Thu Feb 14, 2013 11:59 pm
 
Forum: Molecular Biology
Topic: Two-color in situ help
Replies: 3
Views: 1620

Molecular Cell Biology

... experimentally by incubation of the human A431 epithelial carcinoma cell line with epidermal growth factor (EGF) to measure RME, and horseradish peroxidase (HRP) to measure FPE. A quantitative analysis was carried out by incubating A431 cells with 40 nM ferritin-labelled EGF and 20 μM HRP (which ...

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by Freckles
Wed Dec 07, 2011 11:24 pm
 
Forum: Cell Biology
Topic: Molecular Cell Biology
Replies: 5
Views: 3501

Please Help

The two major enzymes involved are catalase and superoxide dismutase (peroxidase is a family of enzymes). They are involved in beta oxidation and removal of ROS.

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by aptitude
Tue Nov 08, 2011 6:43 am
 
Forum: Cell Biology
Topic: Please Help
Replies: 4
Views: 1656
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