Pepsin A

Then try to cut the bands from gel and identify on MALDI or pepsin digest/LC-MS Allready in progress :) Actually I have found a good question about tricine gel problem in Schägger protocol.. Will try it too.

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Then try to cut the bands from gel and identify on MALDI or pepsin digest/LC-MS

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... Now my problem is that in my school, I lack the proper resources to do a good assay (that is why i switched from pepsin to lactase) and after doing some surfing on the web for good assays, I came across these: "A STANDARD ASSAY MIXTURE for this experiment ...

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... stomach has the ability to detect pH. The actual process is much more complex mechanically, but that doesn't need to brought into the discussion. Pepsin is the active form of a protein called a zymogen which is enzyme that has to be cleaved to be activated. Pepsin as well as other enzymes (enzymes ...

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... proteases. These are enzymes (usually proteins themselves) which snip or chew proteins. Enzymes in the stomach are good examples (e.g. trypsin, pepsin). I don't know what you mean by "simple" proteins- small proteins are usually termed peptides (2-15 amino acids or so), although more ...

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Pepsin A

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MALDI; fusion protein cleaving from a peptide

I need some help...

Re: drink water, orange juice or Cola before eating?

Re: Derived proteins

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Pepsin A

Results from our forum

Re:

MALDI; fusion protein cleaving from a peptide

I need some help...

Re: drink water, orange juice or Cola before eating?

Re: Derived proteins