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Dictionary » P » Pepsin

Pepsin

Definition

noun, plural: pepsins or pepsine

A proteolytic enzyme in the gastric juice of birds, reptiles, fish, and mammals that partially digest dietary proteins into simpler, shorter chains of amino acids such as proteoses and peptones in the presence of hydrochloric acid.


Supplement

Pepsin is capable of digesting many proteins from the food except protamine, keratin, and other highly glycosylated proteins. It is converted from pepsinogen that is released from the gastic pits of the stomach. The conversion of pepsinogen into pepsin occurs in the presence of hydrochloric acid or by the action of another pepsin in the gastric juice.


Word origin: Greek pepsis (digestion), from peptein (to digest)

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Re:

Then try to cut the bands from gel and identify on MALDI or pepsin digest/LC-MS Allready in progress :) Actually I have found a good question about tricine gel problem in Schägger protocol.. Will try it too.

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by Valya
Thu Mar 29, 2012 6:59 pm
 
Forum: Molecular Biology
Topic: MALDI; fusion protein cleaving from a peptide
Replies: 6
Views: 964

MALDI; fusion protein cleaving from a peptide

Then try to cut the bands from gel and identify on MALDI or pepsin digest/LC-MS

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by JackBean
Thu Mar 29, 2012 12:42 pm
 
Forum: Molecular Biology
Topic: MALDI; fusion protein cleaving from a peptide
Replies: 6
Views: 964

I need some help...

... Now my problem is that in my school, I lack the proper resources to do a good assay (that is why i switched from pepsin to lactase) and after doing some surfing on the web for good assays, I came across these: "A STANDARD ASSAY MIXTURE for this experiment ...

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by fubario
Fri Mar 16, 2012 3:34 am
 
Forum: Molecular Biology
Topic: I need some help...
Replies: 3
Views: 1257

Re: drink water, orange juice or Cola before eating?

... stomach has the ability to detect pH. The actual process is much more complex mechanically, but that doesn't need to brought into the discussion. Pepsin is the active form of a protein called a zymogen which is enzyme that has to be cleaved to be activated. Pepsin as well as other enzymes (enzymes ...

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by daniel.kurz
Mon Oct 24, 2011 12:26 am
 
Forum: Physiology
Topic: drink water, orange juice or Cola before eating?
Replies: 13
Views: 28140

Re: Derived proteins

... proteases. These are enzymes (usually proteins themselves) which snip or chew proteins. Enzymes in the stomach are good examples (e.g. trypsin, pepsin). I don't know what you mean by "simple" proteins- small proteins are usually termed peptides (2-15 amino acids or so), although more ...

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by merv
Sun Oct 02, 2011 4:29 pm
 
Forum: Molecular Biology
Topic: Derived proteins
Replies: 3
Views: 2275
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