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Penicillin

penicillin

(Science: drug) probably the best known of the antibiotics, derived from the mould penicillium notatum. It blocks the cross linking reaction in peptidoglycan synthesis and therefore destroys the bacterial cell wall making the bacterium very susceptible to damage. Any of various antibiotics obtained from penicillium molds (or produced synthetically) and used in the treatment of various infections and diseases.An [[antibiotic discovered by the Scottish chemist Sir Alexander Fleming in 1928, which is produced by the mould penicillium that proves [[toxic to a number of [[pathogens and therefore is of excellent medicinal value.


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how to cultrue IEC-6

still now,i found that many paper mention 10%FBS+DMEM,1x105 U/liter of penicillin/streptomycin at 37┬░Cin an atmosphere of 10% CO2 and 95% relative humidity. But in some forum ,someone said,1U/ml insulin must be add into the cultrue medium. Does anyone tell ...

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by lch8934402
Thu Mar 26, 2015 4:05 pm
 
Forum: Cell Biology
Topic: how to cultrue IEC-6
Replies: 1
Views: 15

Methylene Blue Solution use in staining variety of bacteria?

... positive bacteria, such as those found in yogurt (Lactobacillus and Streptococcus) are less pathogenic. Their cell walls can be disrupted with penicillin or with lysozyme (an enzyme that our cells are able to produce). Gram negative bacteria, such as E.coli, Helicobacter, and other bacteria ...

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by scytek
Sun Feb 22, 2015 9:22 am
 
Forum: General Discussion
Topic: Methylene Blue Solution use in staining variety of bacteria?
Replies: 0
Views: 271

Bacteremia in humans

... bacteria. E. coli and S. aureus are on my potential bacteria. The six antibiotics are ampicillin, streptomycin, chloramphenicol, sulphatriad, penicillin G and tetracycline. Now, I know EC and SA are big villains, however, I have issues finding exactly how big. That is, I can't find stats that ...

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by trivedv
Thu May 02, 2013 11:56 am
 
Forum: Human Biology
Topic: Bacteremia in humans
Replies: 1
Views: 1961

Why dialyse DMEM before protein purification (Ni-Affinity)?

... with high glucose and L-Glutamin with 5% fetal calf serum and 5% penicillin/streptomycin. Or do you mean anything else?

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by LittleBird
Sun May 20, 2012 5:55 pm
 
Forum: Molecular Biology
Topic: Why dialyse DMEM before protein purification (Ni-Affinity)?
Replies: 5
Views: 3992

Why dialyse DMEM before protein purification (Ni-Affinity)?

... transfected HEK293T in order to express a his-tagged protein. The cells were kept in DMEM (high glucose, modified with 5% FCS and 1% streptomycin/penicillin), which was collected daily and exchanged with fresh modified DMEM. The collected supernatants where then dialysed against a his-dialysis-buffer ...

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by LittleBird
Sun May 20, 2012 3:43 pm
 
Forum: Molecular Biology
Topic: Why dialyse DMEM before protein purification (Ni-Affinity)?
Replies: 5
Views: 3992
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