Dictionary » P » Pellet

Pellet

pellet

1. A pilule, or very small pill.

2. A small rod-shaped or ovoid dosage form that is sterile and is composed essentially of pure steroid hormones in compressed form, intended for subcutaneous implantation in body tissues; serves as a depot providing for the slow release of the hormone over an extended period of time.

Origin: Fr. Pelote; L. Pila, a ball


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A dilution question about RNA

Hello, I have a basic and probably silly question. I am isolating RNA with TRIzol. I have performed the basic steps and resolve the pellet in 50 uL water. I ve measured the concentration and it was 3313 ng/uL (Around 165 ug). Now my next step is DNase treatment. It says 1 unit of enzyme ...

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by golgicik
Mon Feb 03, 2014 6:30 pm
 
Forum: Molecular Biology
Topic: A dilution question about RNA
Replies: 1
Views: 831

Re: centrifugation of hemocytes

I can't see any pellet, BUT today it tried again, after 24h of incubation (cells have time to adhere), and centrifugation at 400 for 5 min, I gently took off the medium and resuspend the cells by pipetting thoroughly up/down: the ...

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by Thom
Tue Jan 28, 2014 4:16 pm
 
Forum: Cell Biology
Topic: centrifugation of hemocytes
Replies: 5
Views: 2914

Re: centrifugation of hemocytes

Before you wash the cells, can you see any pellet? Wash gently, add your final PBS and pipet to resuspend. Your protocol sounds good to me.

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by jonmoulton
Tue Jan 28, 2014 3:53 pm
 
Forum: Cell Biology
Topic: centrifugation of hemocytes
Replies: 5
Views: 2914

centrifugation of hemocytes

... of centrifugation because i want my cells in viable status... I can't figure out where my trouble comes from. Apparently it is not enough to have pellet that will not detach after a PBS-washing step … but many article do not report that, so the problem is from protocol. Any ideas?? Many thanks ...

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by Thom
Mon Jan 27, 2014 6:41 pm
 
Forum: Cell Biology
Topic: centrifugation of hemocytes
Replies: 5
Views: 2914

Getting Bacteria from an OD0.8 to an OD 0.1

... been doing in the past but just want to confirm that what I've been doing in correct. So from my 50mL, I took 1mL, spun and then resuspended the pellet in 1mL of PBS. (I now have 1mL of bacteria at OD0.8 in PBS) I took 125uL from this and added to 875uL of PBS (OD has gone down 8x now) Is this ...

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by biology_06er
Sun Jun 16, 2013 12:15 pm
 
Forum: Microbiology
Topic: Getting Bacteria from an OD0.8 to an OD 0.1
Replies: 1
Views: 1053
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