Dictionary » P » Pellet

Pellet

pellet

1. A pilule, or very small pill.

2. A small rod-shaped or ovoid dosage form that is sterile and is composed essentially of pure steroid hormones in compressed form, intended for subcutaneous implantation in body tissues; serves as a depot providing for the slow release of the hormone over an extended period of time.

Origin: Fr. Pelote; L. Pila, a ball


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western blotting of DNA-binding protein of mammalian cell

... 5 min, followed by passing the lysate through 24 G needle about 5 times...then centrifuged at 12000 g for 15 min..collected the supernatant and pellet.. from the supernatant 40 ul of sample taken and 2X sample buffer added; to the pellet 50ul of 2X sample buffer added, boiled for 5 min in 95C ...

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by drelamuruganvet
Mon Jan 19, 2015 9:39 am
 
Forum: Molecular Biology
Topic: western blotting of DNA-binding protein of mammalian cell
Replies: 0
Views: 393

A dilution question about RNA

Hello, I have a basic and probably silly question. I am isolating RNA with TRIzol. I have performed the basic steps and resolve the pellet in 50 uL water. I ve measured the concentration and it was 3313 ng/uL (Around 165 ug). Now my next step is DNase treatment. It says 1 unit of enzyme ...

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by golgicik
Mon Feb 03, 2014 6:30 pm
 
Forum: Molecular Biology
Topic: A dilution question about RNA
Replies: 1
Views: 1559

Re: centrifugation of hemocytes

I can't see any pellet, BUT today it tried again, after 24h of incubation (cells have time to adhere), and centrifugation at 400 for 5 min, I gently took off the medium and resuspend the cells by pipetting thoroughly up/down: the ...

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by Thom
Tue Jan 28, 2014 4:16 pm
 
Forum: Cell Biology
Topic: centrifugation of hemocytes
Replies: 5
Views: 5112

Re: centrifugation of hemocytes

Before you wash the cells, can you see any pellet? Wash gently, add your final PBS and pipet to resuspend. Your protocol sounds good to me.

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by jonmoulton
Tue Jan 28, 2014 3:53 pm
 
Forum: Cell Biology
Topic: centrifugation of hemocytes
Replies: 5
Views: 5112

centrifugation of hemocytes

... of centrifugation because i want my cells in viable status... I can't figure out where my trouble comes from. Apparently it is not enough to have pellet that will not detach after a PBS-washing step … but many article do not report that, so the problem is from protocol. Any ideas?? Many thanks ...

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by Thom
Mon Jan 27, 2014 6:41 pm
 
Forum: Cell Biology
Topic: centrifugation of hemocytes
Replies: 5
Views: 5112
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