Peak

In AQ mode one can design point mutation detecting dispensation orders and peak height ration will tell the prevalence of mutation in a wild type background.

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... to be transmitted to a new species when the fitness valley is shallow? Yes. The location of the organisms in a species tend to concentrate at the peak, but mutations and sexual reproduction keep it spread out around the peak, not all at the peak. For a shallow valley, its more likely that this ...

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... I did MLR proliferation assay with 10micromole of CFSE and did not see any division till day 5 but there was a slight movement in the original peak. On day 8 i saw a popultion which was completely negative for the CFSE and also one small peak which was slightly positive for CFSE but the cells ...

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... marine species using QIAGEN RNeasy Plus mini kit eluted with 40ul RNase free water. Nanodrop gives a low reading and a weak absorption peak (~10ng/ul). Gel analysis of 3ul such RNA yielded no bands (not even the ribosomal RNAs). The RNA sample have been heated at 65C for 5min before ...

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How does the single peak relate to your negative control? Do all your cells have full or lowered CFSE intensity? Does the intensity go down each day? Having too much CFSE should not be the cause, because even high concentrations diminish ...

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