Dictionary » P » Pcr

Pcr

PCR --> polymerase chain reaction

(Science: molecular biology, technique) The first practical system for in vitro amplification of dNA and as such one of the most important recent developments in molecular biology.

Two synthetic oligonucleotide primers, which are complementary to two regions of the target dNA (one for each strand) to be amplified, are added to the target DNA (that need not be pure), in the presence of excess deoxynucleotides and taq polymerase, a heat stable dNA polymerase. In a series (typically 30) of temperature cycles, the target DNA is repeatedly denatured (around 90_C), annealed to the primers (typically at 50-60_C) and a daughter strand extended from the primers (72_C). As the daughter strands themselves act as templates for subsequent cycles, DNA fragments matching both primers are amplified exponentially, rather than linearly.

The original dNA need thus be neither pure nor abundant and the polymerase chain reaction has accordingly become widely used not only in research, but in clinical diagnostics and forensic science.

Acronym: PCR


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


Gateway technology

... you get your gene into an universal vector from which you can easily transfer it into variety of destination vectors without any need of further PCR as PCR is often troublesome and introduces mutations.

See entire post
by JackBean
Wed Aug 20, 2014 6:42 pm
 
Forum: Molecular Biology
Topic: Gateway technology
Replies: 1
Views: 91

Gateway technology

... bacterias. However, why do I need to perform the BP reaction, if I want to obtain an expression clone of a certain gene? Why can't I use an attL-PCR product or a linearized attL DNA and make a reaction with the destination vector, therefore obtaining the expression clone attB in the end? I can't ...

See entire post
by miibay
Tue Aug 19, 2014 10:47 pm
 
Forum: Molecular Biology
Topic: Gateway technology
Replies: 1
Views: 91

Re: Totipotent Stem Cell From Molecular Components

... particular protein is the average of the gene expression in # of strands of mRNA * 100. I am getting the protein this way. I take the results of PCR and put it in a test tube with RNA polymerases, Ribosomes, and other proteins that help with protein synthesis. I then look at the structure of ...

See entire post
by caters
Fri Aug 01, 2014 1:42 am
 
Forum: Cell Biology
Topic: Totipotent Stem Cell From Molecular Components
Replies: 2
Views: 2374

Re: An interesting PCR smearing...I dont know what to do :(

... very well ( a single bright band ) but the other two exon amplification is not coming properly.and this has occured recently. ( even though the PCR reaction was standardized earlier ) I have used same reagents , pipettes , PCR machine , reconstituted new primers for amplifying all 3 exons . ...

See entire post
by priyah
Sat Jun 28, 2014 5:20 pm
 
Forum: Molecular Biology
Topic: An interesting PCR smearing...I dont know what to do :(
Replies: 9
Views: 6280

Re: Totipotent Stem Cell From Molecular Components

... and how will you get the protein? Are you really sure that the stemm cells contain all protein in the same amount? A4: amplicon is the result of PCR. You need some template though, in your case probably the DNA.

See entire post
by JackBean
Tue May 13, 2014 5:04 pm
 
Forum: Cell Biology
Topic: Totipotent Stem Cell From Molecular Components
Replies: 2
Views: 2374
View all matching forum results

This page was last modified 21:16, 3 October 2005. This page has been accessed 16,651 times. 
What links here | Related changes | Permanent link