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Patch clamp

patch clamp --> patch clamping

(Science: physiology) A specialised and powerful variant of voltage clamping, in which a patch electrode of relatively large tip diameter (5m) is pressed tightly against the plasma membrane of a cell, forming an electrically tight, gigohm seal.

The current flowing through individual ion channels can then be measured. Different variants on this technique allow different surfaces of the plasma membrane to be exposed to the bathing medium: the contact just described is a cell attached patch.

If the electrode is pulled away, leaving just a small disc of plasma membrane occluding the tip of the electrode, it is called an inside out patch.

If suction is applied to a cell attached patch, bursting the plasma membrane under the electrode, a whole cell patch (similar to an intracellular recording) is formed.

If the electrode is withdrawn from the whole cell patch, the membrane fragments adhering to the electrode reform a seal across the tip, forming an outside out patch.


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Patch-Clamping - Need help in basics

... interaction, but at the moment I have to deal with some results of patch-clamp recordings. Unfortunately I've never been taught how to interpret those data, and it ...

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by Jennileinchen
Mon Jul 22, 2013 3:22 pm
 
Forum: Molecular Biology
Topic: Patch-Clamping - Need help in basics
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neuro questions!

Whole-cell patch clamp electrophysiology can be used to record: a) the activity of the ion channels that are present in the patch ...

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by yj90
Wed Jan 19, 2011 4:37 am
 
Forum: Physiology
Topic: neuro questions!
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Sugar Allergy

... of peppermint oil on gastro-intestinal smooth muscle. An analysis using patch clamp electrophysiology and isolated tissue pharmacology in rabbit and guinea pig. Gastroenterology ...

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Sat Jan 12, 2008 4:20 pm
 
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Cell Manipulation Question

... and isolate and count specific cells. As for single cells one could use patch-clamp methods (though this is usually used for measureing ion channels)

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by Vagabond
Mon Feb 26, 2007 11:21 pm
 
Forum: Cell Biology
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