Parallel

if it's from the opposite strand, it must be antiparallel. If it's parallel, it must be transcribed either from the mRNA or from other locus.

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... miRNA expresses, sequence analysis revealed it possibly transcribes from the opposite strand. sequence analysis further confirmed that it would be parallel to our desire mRNA. Now i need to know do they bind?

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Ah, jute, sentimental reminiscences... OK, so you know that it is expressed from the other strand, but for some reason you think it's parallel?

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... under different stress conditions at different hours. Now as it expresses from the reverse orientation of our gene, it would be complementary but parallel to our gene. Do they bind with each other. If they dont bind then how can i confirm the target gene of this miRNA? For your convenience i would ...

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I agree with Jack. Polymerase adds bases by elongating from the 3' end of a nucleic acid strand which is bound in an antiparallel orientation with the template strand. I have not heard of priming the template backwards (parallel). I have never heard of a natural system making parallel ...

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