Paraformaldehyde

Could any one let me know what a cell fixation process exactly do to the cells? Does it crosslink the proteins? If I fix cells using paraformaldehyde will the surface expressed receptors get denatured?

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... generalized procedure for immunofluorescence labeling of adherent cell cultures involves fixation of the cells with a suitable reagent, such as paraformaldehyde, glutaraldehyde, acetone, or methanol. The organic solvents serve double duty by also removing most of the plasma membrane components, ...

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I want to know if the action of PFA (paraformaldehyde) in immunoflurescence protocoles is only fixing celles or has it a permeabilisation action. Khanhs for Help :?: :?:

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