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Paraffin

paraffin

(Science: chemical) A mixture of solid hydrocarbons obtained from petroleum. It has a wide range of uses including as a stiffening agent in ointments, as a lubricant, and as a topical anti-inflammatory. It is also commonly used as an embedding material in histology.

Pharmacological action: anti-inflammatory agent, topical, pharmaceutic aid, sclerosing solutions.

Chemical name: paraffin waxes and Hydrocarbon waxes


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identify bacterias in Gram stain kit &The Giemsa Stain Kit

... for use in the visualization of cells present in hematopoietic tissues and certain microorganisms. This kit may be used on formalin-fixed, paraffin-embedded or frozen sections. Nuclei: Blue/Violet Cytoplasm: Light Blue Collagen: Pale Pink Muscle Fibers: Pale Pink Erythrocytes: Gray, Yellow ...

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by scytek
Sat Mar 15, 2014 1:11 pm
 
Forum: General Discussion
Topic: identify bacterias in Gram stain kit &The Giemsa Stain Kit
Replies: 0
Views: 1416

embedding cells in paraffin

Hi, How can I embed cells in a 6-wells plate into paraffin wax? I cannot detach the cells so this has to be done in situ. Thanks.

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by aguy
Thu Jan 24, 2013 11:19 pm
 
Forum: General Discussion
Topic: embedding cells in paraffin
Replies: 0
Views: 812

Microorganisms and organic preservation?

... once water is changed for organic solvent, you can change organic solvent for some organic chemical that will solidify. For example hot paraffin. However, I'm not sure about colors-pigments could be washed away by organic solvents.

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by triton
Wed Aug 22, 2012 7:01 pm
 
Forum: Microbiology
Topic: Microorganisms and organic preservation?
Replies: 1
Views: 3810

DNA Extraction/ Amplication

... and phenol-chloroform method was used to extract DNA in whole blood, fresh tissues, frozen tissues embedding with OCT and tissues embedding in paraffin. It results that 200-300 microg DNA was extracted from 1 ml whole blood or 0.2 g fresh tissues or frozen tissue embedding with OCT. DNA extracted ...

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by brandi21
Fri Aug 26, 2011 11:41 am
 
Forum: Molecular Biology
Topic: DNA Extraction/ Amplication
Replies: 2
Views: 7670

Re: My new experiment in DNA modification

Okay, I'm having some problems with the Qiagen kit. Ive got frozen paraffin embedded tissues and modified the extracted dna. i ran two pcr amplifications at about 300bp and then 200bp. dna recovery is low after the bisulfite modification. My samples are showing ...

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by sara135
Tue Jul 12, 2011 1:50 pm
 
Forum: General Discussion
Topic: My new experiment in DNA modification
Replies: 7
Views: 4524
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