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P face

P face --> freeze fracture

method of specimen preparation for the electron microscope in which rapidly frozen tissue is cracked so as to produce a fracture plane through the specimen. The surface of the fracture plane is then shadowed by heavy metal vapour, strengthened by a carbon film and the underlying specimen is digested away, leaving a replica that can be picked up on a grid and examined in the transmission electron microscope. The great advantage of the method is that the fracture plane tends to pass along the centre of lipid bilayers and it is therefore possible to get en face views of membranes that reveal the pattern of integral membrane proteins. The e face is the outer lamella of the plasma membrane viewed as if from within the cell, the p face the inner lamella viewed from outside the cell. Fracture planes also often pass along lines of weakness such as the interface between cytoplasm and membrane, so that outer and inner membrane surfaces can be viewed. Further information about the structure can be revealed by freeze etching. Extremely rapid freezing followed by deep etching has allowed the structure of the cytoplasm to be studied without the artefacts that might be introduced by fixation.


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Changes on our face when we grow

What changes does parts of our face make when we grow up ? Lets see on nose, eye or ear ? Can we find out the age of person by measuring or seeing any parts of any person ??

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by sshasi009
Thu Mar 13, 2014 5:20 am
 
Forum: Human Biology
Topic: Changes on our face when we grow
Replies: 1
Views: 81

Re:

... both zoological and botanical, as per the two-kingdom system. Also, will you please ask them what ever happened to "socialism with a human face"? "Savage capitalism" is now everywhere and getting rid of the "useless eaters" as though they were algae, slime molds ...

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by JackBean
Mon Mar 03, 2014 1:11 pm
 
Forum: Molecular Biology
Topic: Newbie question about the first life
Replies: 14
Views: 717

Newbie question about the first life

... both zoological and botanical, as per the two-kingdom system. Also, will you please ask them what ever happened to "socialism with a human face"? "Savage capitalism" is now everywhere and getting rid of the "useless eaters" as though they were algae, slime molds ...

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by piscilactovegetarian
Sat Mar 01, 2014 12:28 pm
 
Forum: Molecular Biology
Topic: Newbie question about the first life
Replies: 14
Views: 717

Re: Am I the Next Step in Human Evolution?

... a lot of hair who look younger than they are. Example, my brother grows a very thick beard and has a lot of hair on his chest, arms, legs, but his face is very young looking, not matching his numerical age of 26. Also, the fact that you only look a few years off your actual age cannot be used to ...

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by badgertoes
Sat Dec 28, 2013 8:31 am
 
Forum: Evolution
Topic: Am I the Next Step in Human Evolution?
Replies: 23
Views: 23654

Re: Human Collembola Infestations

... out of my skin. It is driving me nuts. I bought sulfur shampoo and ointment and only helped a little. I feel them coming out of the skin on my face too. What are these things? Please someone e-mail me at [email protected] and help me with a remedy on how to kill these things. Is this ...

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by sunshine123
Thu Dec 26, 2013 5:59 pm
 
Forum: Human Biology
Topic: Human Collembola Infestations
Replies: 40
Views: 91388
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