P-r intervals

... life of my protein of interest, I treated my cells with cycloheximide(CHX), which is an eukaryotic translation inhibitor, through different time intervals. Then, I looked at the protein amounts by Western Blot. However, I saw that as I left them in medium containing CHX more, the levels of my ...

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... yes because neurones are insulated ( some are anyway) with a substance called myelin this enables very fast transmission and has gaps at regular intervals called nodes of ranvier where diffusion of ions can occur. If we had lots of axons there would be loads of gaps and the action potentials ...

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... the size of my marked RNA 5. Add RNA to mammalian cell extracts, e.g. HeLa 6. While the RNA undergoes maturing, I collect samples with regular intervals of time 7. Use phenol to extract proteins from each sample 8. Run MALDI-MS starting with 0 minutes sample. After that, continue with the next ...

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... report at university. We done an experiment on two cultures (L and M) measuring the growth using a spectrophotometer set at OD600 at set time intervals (every 30mins, 0-150). We then had to find and plot the natural log, these were my results (obviously not on a graph): Time Culture L lnCulture ...

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... field, but I'd guess you could do it so that you grow your cells under conditions where they form collagen and then take some cells at regular intervals (depending on the production speed you could take, say, one sample every two hours for 24 hours, or whatever that seems useful) and stain ...

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