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P-k antibodies


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Re: Difference between ICC and IF

This is how immunohistochemistry is typically done, so that is probably enough of a name. However, you could specify the antibodies, for example: immmunihistochemistry using goat anti-VEGFR primary antibody and anti-goat-IgG secondary antibody conjugated to horseradish peroxidase.

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by jonmoulton
Mon Feb 25, 2013 5:11 pm
 
Forum: Molecular Biology
Topic: Difference between ICC and IF
Replies: 7
Views: 7531

Re: Re:

... be developed. If I had to guess, I would perhaps go for monoclonal antibody-based treatments to be the next big thing. The specific nature of antibodies would allow targeting only the cancer cells without harming the healthy cells, and the antibody can stimulate the body's own immune cells ...

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by Ahsmeah
Tue Feb 05, 2013 10:10 pm
 
Forum: Physiology
Topic: Cancer = Mutation: Stopping Mutation Would Cure Cancer?
Replies: 6
Views: 1029

Re: Re:

... be developed. If I had to guess, I would perhaps go for monoclonal antibody-based treatments to be the next big thing. The specific nature of antibodies would allow targeting only the cancer cells without harming the healthy cells, and the antibody can stimulate the body's own immune cells ...

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by biohazard
Tue Feb 05, 2013 1:23 pm
 
Forum: Physiology
Topic: Cancer = Mutation: Stopping Mutation Would Cure Cancer?
Replies: 6
Views: 1029

nuclear DNA binding

... at all. Is there a standard that I might not be aware of? I thought that preparing nuclear extracts, performing IP against the protein (many antibodies are available) and checking DNA levels in the pull-downed material against negative (matrix protein) and positive control (like Creb) would ...

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by Balls
Tue Jan 22, 2013 9:03 am
 
Forum: Molecular Biology
Topic: nuclear DNA binding
Replies: 1
Views: 300

Re: Cytokine - Receptor concepts

... you can do some biological experiments to determine the localization and distribution of your interested receptors with purchased specifc antibodies. For example, immunohistochemistry is a useful rechnique. To get a reference, you can visit http://www.immunohistochemistry.us/

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by sunhan1985
Fri Dec 21, 2012 2:27 am
 
Forum: Cell Biology
Topic: Cytokine - Receptor concepts
Replies: 7
Views: 10376
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