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Overlay

overlay

1. To lay, or spread, something over or across; hence, to cover; to overwhelm; to press excessively upon. When any country is overlaid by the multitude which live upon it. (Sir W. Raleigh) As when a cloud his beams doth overlay. (Spenser) Framed of cedar overlaid with gold. (milton) And overlay With this portentous bridge the dark abyss. (Milton)

2. To smother with a close covering, or by lying upon. This woman's child died in the night; because she overlaid it. (1 kings III. 19) A heap of ashes that o'erlays your fire. (Dryden)

3. To put an overlay on.

Origin: Overlaid; Overlaying.


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High variation among triplicates in ELISA

... as the capture Ag, anti IFNgamma as the antibody and a 1:6000 diluted Protein-A HRP which serves as the detecting factor. ON top of that, I overlay with a KPL ECL mix (which I know to work excellently in other assays I've done with it). What I've seen is usually a variation of 1 out of 3 ...

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by pilpel
Mon Mar 10, 2008 2:04 pm
 
Forum: Molecular Biology
Topic: High variation among triplicates in ELISA
Replies: 1
Views: 1464

Plaque assay

... that it is a negative result but what does that mean??? Also....a question reads " why was it important to use a hard agar with a soft agar overlay instead of vice versa???" Thank you

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by csumicroa
Thu Dec 14, 2006 2:43 am
 
Forum: Microbiology
Topic: Plaque assay
Replies: 1
Views: 3867

The Fiber Disease

... Oscar de la Renta, Stephen Burrows, Elisa Jimenez and others included a strapless beige ball gown, a cream baby-doll dress with ribbon and sheer overlay, and a pink and yellow taffeta skirt with a silver recycled polyester bustier. Christopher Ryan, chief technology officer of Natureworks LLC, ...

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by London
Fri Aug 11, 2006 2:13 pm
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 5559946


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