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Overlapping

overlapping

(Science: cell biology) In cell locomotion situation in which the leading lamella of one cell moves actively over the dorsal surface of another cell should be distinguished from underlapping.


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Gene knockdowns in the wasp Nasonia vitripennis

... genes ..., it often does not provide significant knockdown of later-acting genes. To overcome this limitation, we designed an Nv eve morpholino overlapping the translation start site, as well as one directed at the exon-intron junction in the homeobox. These two independent morpholinos are expected ...

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by jonmoulton
Fri Mar 07, 2014 5:57 pm
 
Forum: Molecular Biology
Topic: Gene knockdowns in the wasp Nasonia vitripennis
Replies: 0
Views: 1162

cell specific markers

... significant part of the aggressor’s population would be potentially vulnerable. In addition, the P450 system comprises many dozens of enzymes with overlapping activities. Targeting a chemical or biological compound to one specific P450 enzyme would be very challenging. A systematic search in two ...

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by MarkHolland
Sat Mar 30, 2013 3:16 pm
 
Forum: Cell Biology
Topic: cell specific markers
Replies: 3
Views: 6278

Re:

... at a time? Is your aim to clone all variants or are you fine with only some? Well, we initially had 4 sets of primers. I was going to amplify 4 overlapping regions of the gene, run them on a gel, cut out the bands of expected size, purify them, and eventually sequence and ligate them together ...

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by polkadotshorts
Mon Dec 10, 2012 7:12 pm
 
Forum: Genetics
Topic: How to clone a gene with lots of splicing intermediates?
Replies: 6
Views: 3386

Re: How do I create the sequence contig of this DNA?

Look for overlapping sequences. For example, Read 1 begins with tattat. Does this sequence occur in any of the other reads?

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by wbla3335
Tue Nov 20, 2012 7:14 pm
 
Forum: Genetics
Topic: How do I create the sequence contig of this DNA?
Replies: 2
Views: 1665

Re: question for evolutionary biologist.

... contains added watermark sequences for identification of the genome as synthetic and also antibiotic resistance markers to allow for selection. Overlapping cassettes of 1080 were assembled from chemically synthesized oligonucleotides. The complete synthetic genome was assembled by transformation ...

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by scottie
Thu Sep 13, 2012 1:22 pm
 
Forum: Evolution
Topic: question for evolutionary biologist.
Replies: 26
Views: 28516
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