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Overlap hybridization

overlap hybridization --> chromosome walking

A procedure to find and sequence a gene whose approximate position in a chromosome is known by classical genetic linkage studies. Starting with the known sequence of a gene shown by classical genetics to be near to the novel gene, new clones are picked from a genomic library by hybridisation with a short probe generated from the appropriate end of the known sequence. The new clones are then sequenced, new probes generated and the process repeated until the gene of interest is reached.


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Primary lambda phage screen - precipitated probe?

... both films are spotty to the same degree, few (if any) of the spots overlap. Going back to the procedure, I boiled the labeled probe (32P dCTP-labelled ... it on ice for a few minutes (to minimize renaturation) and added 1 mL hybridization buffer (6x SSC, 10x Denhardt's, 2% SDS, 0.2 mg/mL sheared ...

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by icequeen11
Fri Jun 15, 2007 7:15 pm
 
Forum: Molecular Biology
Topic: Primary lambda phage screen - precipitated probe?
Replies: 1
Views: 1681

In response to your PM to me

... completely yellow. What is the difference between cross pollination and hybridization? Perhaps it is only cross pollination that we are observing ... NATURALLY in the wild where the ranges of two closely related species overlap (Dryopteris x australis).” Looks like they can. :wink: Peace, Sabrina

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by Sabrina
Fri Jun 23, 2006 8:26 am
 
Forum: Botany Discussion
Topic: what is this flower please?
Replies: 2
Views: 1613

Re: In regards to 'species'

... Many pairs of species remain distinct despite a small amount of hybridization between them. Some groups are asexual or frequently produce ... concepts, for modeling and explaining. There is a great deal of overlap between the two purposes, but a definition that serves one is not ...

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by clarence
Thu May 19, 2005 12:07 pm
 
Forum: Zoology Discussion
Topic: In regards to 'species'
Replies: 10
Views: 8691

Ligers and Tigons oh my!

... biochemical, and molecular markers reveal that modern and ancient hybridization affects the distribution of genetic variation (Critchfield ... and continual dispersal of parental genotypes into the zone of overlap. As long as there is an overlap in mating zones hybrids will go on ...

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by Inuyasha
Tue May 10, 2005 9:55 pm
 
Forum: Zoology Discussion
Topic: Ligers and Tigons oh my!
Replies: 111
Views: 156369


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