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Dictionary » O » Overhang

Overhang

Definition

noun

(molecular biology) Unpaired nucleotide(s) in the end of a DNA molecule, which may be produced by a staggered cut on the DNA using a restriction enzyme.

(dentistry) An excess of filling material projecting beyond the cavity margin or normal tooth contour.


verb

To hang across or beyond; be suspended over.


Supplement

In molecular biology, an overhang can be in either strand of a DNA molecule. Thus, a 3' or 5' overhang may be produced. An overhang may only be a single unpaired nucleotide in the end of DNA or a longer stretch of unpaired nucleotides like in cohesive end.


Related forms: overhangs (verb: simple present), overhanging (verb: present participle), overhung (verb: past, past participle).

Compare: blunt end (molecular biology).
See also: sticky end, restriction enzyme.


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Designing primers to clone into expression vector

Hmmm... I would say create 2 primers that cover the end of your sequence of iterest and add a restriction site of your choice as an overhang at each end.

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by canalon
Thu Feb 16, 2012 3:08 am
 
Forum: Molecular Biology
Topic: Designing primers to clone into expression vector
Replies: 1
Views: 860

LIC PCR Primer with Signal Peptide?

... system with transport, it can be mislocated. Of course, the primer won't bind on the START codon. Imagine it in two parts I unaligning part (the overhang + ATG and maybe even some more nucleotides) II aligning part (the part which aligns behind the signal peptide) - this part must be long enough ...

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by JackBean
Wed Oct 19, 2011 6:54 pm
 
Forum: Molecular Biology
Topic: LIC PCR Primer with Signal Peptide?
Replies: 3
Views: 1195

LIC PCR Primer with Signal Peptide?

... fit the vector so this primer (~25 bp) will be extremely specific. SO i don't understand how we can have the (idealised) forward primer form: <overhang code to fit pET vector> <ATG><remove the 78 nucleotides of the signal peptide><the rest of my primer> Presuming the signal ...

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by stalinsma
Wed Oct 19, 2011 6:03 pm
 
Forum: Molecular Biology
Topic: LIC PCR Primer with Signal Peptide?
Replies: 3
Views: 1195

PLease Please I need help with my PCR! Greatly appreciated!

Shouldn't you have some overhang on the primers, so that the RE will be able to cut your product? As of the PCR, I would just cut the lower band and not bother with the rest. What is your template?

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by JackBean
Tue Aug 30, 2011 6:06 am
 
Forum: Molecular Biology
Topic: PLease Please I need help with my PCR! Greatly appreciated!
Replies: 8
Views: 7409

Re: restriction enzyme question.

... made...the Taq polymerase will only extend from the 3 end...on one website I read: During the first round of PCR the complimentary strand of the overhang will be added, and in subsequent rounds of PCR this site will be amplified along with the rest of your PCR product. In the end, you will have ...

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by biology_06er
Sun Jun 19, 2011 1:28 pm
 
Forum: General Discussion
Topic: restriction enzyme question.
Replies: 9
Views: 3214
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