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Ovalbumin

Definition

noun

(biochemistry) The water-soluble albumin which is the primary component of the egg white (about 60-65% of the total protein).


Supplement

The biological function of ovalbumin is unclear although it is said to act as a storage protein. In research, the ovalbumin (of chicken egg) is used as a molecular weight marker for calibrating electrophoresis gels. It is also used to stimulate allergic reaction in test subjects. In medicine, it is administered to patients suspected of being poisoned by heavy metals (e.g. iron).


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How to draw graphs for SDS PAGE results?

Ive got one gel with molecular weight marker: Phosphorylase B, BSA, Ovalbumin, Carbonic anhydrase, Lactoglobulin and Aprotinin. Then ive got 4 gels with same protein of 150 Kda giving two bands of 50 KDa and 25 KDa. is there anyway i can draw graphs from this ...

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by craterblizz
Thu Feb 23, 2012 3:47 pm
 
Forum: General Discussion
Topic: How to draw graphs for SDS PAGE results?
Replies: 4
Views: 2011

Re: Determining an Ig percentage from crude serum w/ELISA

... antibody. The OVA-IgE uses a monoclonal antibody to mouse IgE heavy chain attached to the wells as a capture antibody and then biotinylated ovalbumin and streptavidin-peroxidase in the presence of substrate is used as a detection antibody. If you go to each on our website you can view the ...

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by researcher4life
Fri May 07, 2010 6:42 pm
 
Forum: Molecular Biology
Topic: Determining an Ig percentage from crude serum w/ELISA
Replies: 2
Views: 1724

Re: thio groups in ovalbumin

My name is Dr. Ros Dickison. I determined the number of thio groups in ovalbumin as part of my Masters thesis. I continued the study and in my doctoral dissertation found that when ovalbumin is dephosphorylated the numer of thio groups change indicating a conformational ...

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by sdekivit
Wed Jan 25, 2006 9:20 am
 
Forum: Molecular Biology
Topic: Determining thiol groups...??
Replies: 5
Views: 6069

thio groups in ovalbumin

My name is Dr. Ros Dickison. I determined the number of thio groups in ovalbumin as part of my Masters thesis. I continued the study and in my doctoral dissertation found that when ovalbumin is dephosphorylated the numer of thio groups change indicating a conformational ...

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by ros
Mon Jan 23, 2006 7:32 pm
 
Forum: Molecular Biology
Topic: Determining thiol groups...??
Replies: 5
Views: 6069

Determining thiol groups...??

First we did the purification process of the ovalbumin. Then we determined the absorbances of native and purified ovalbumin, both with and without SDS and using DTNB in the samples to do it. We also took readings of the native and our sample ovalbumin ...

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by sdekivit
Mon Jan 02, 2006 11:33 am
 
Forum: Molecular Biology
Topic: Determining thiol groups...??
Replies: 5
Views: 6069
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