Osmotic shock

... hypertonic to the left side, meaning the right side has more solute Iso osmotic however compares many solutes at one time in an interacting environment. ... this for better of worse please do so as I also have exams......... :shock:

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... is to dilute and remove the DMSO, but not to do it too fast because the osmotic shock will lyse the cells. Hope this helps!

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... it out then add again etc with a step of adding cresyl violet..so is osmotic shock still the case?

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Just plain water? Maybe osmotic shock.

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... of the main problems during thawing is not the crystal formation, but osmotic shock; too fast dilution of the cryoprservant may damage the cells. In some protocols glycerol ...

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