Dictionary » O » Orientation chamber

Orientation chamber

orientation chamber

(Science: cell culture) chamber designed by Zigmond in which to test the ability of cells (neutrophils) to orient in a gradient of chemoattractant. The chamber is similar to a haemocytometer, but with a depth of only ca. 20m. The gradient is set up by diffusion from one well to the other and the orientation of cells towards the well containing chemoattractant is scored on the basis of their morphology or by filming their movement.

Please contribute to this project, if you have more information about this term feel free to edit this page

Results from our forum

biology urgent need help?

... whether a particular insect prefer a dark or a bright environment. A chamber with a gradient in light intensity from one end (dark) to the other ... confounding variables? A - Varying humidity across the chamber B - The orientation of the chamber C - The age of the insects D - The non-reflective ...

See entire post
by zak
Sun Dec 15, 2013 12:19 pm
Forum: General Discussion
Topic: biology urgent need help?
Replies: 3
Views: 2717

E. Coli glycerol stock cytometry

... recently been trying to count E. coli using a Petroff-Hauser counting chamber. I wanted to count cells straight out of my glycerol stocks, but ... cells look elongated and many appear to be fused together in a linear orientation. Often times, several appear to be fused in chains of 5 or 6 ...

See entire post
by Leon Fnord
Wed Nov 28, 2007 11:35 pm
Forum: Microbiology
Topic: E. Coli glycerol stock cytometry
Replies: 0
Views: 2735

This page was last modified 21:16, 3 October 2005. This page has been accessed 837 times. 
What links here | Related changes | Permanent link