(Science: cell culture) chamber designed by Zigmond in which to test the ability of cells (neutrophils) to orient in a gradient of chemoattractant. The chamber is similar to a haemocytometer, but with a depth of only ca. 20m. The gradient is set up by diffusion from one well to the other and the orientation of cells towards the well containing chemoattractant is scored on the basis of their morphology or by filming their movement.
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... recently been trying to count E. coli using a Petroff-Hauser counting chamber. I wanted to count cells straight out of my glycerol stocks, but ... cells look elongated and many appear to be fused together in a linear orientation. Often times, several appear to be fused in chains of 5 or 6 ...
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