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Organ culture

organ culture

(Science: cell biology) culture in vitro of pieces of tissue (as opposed to single cells) in such a way as to maintain some normal spatial relationships between cells and some normal function. Contrast with tissue culture.


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Help with Xenotransplantation please.

... plasmid. Afaik, the modified cells themselves are rarely grown in a culture for somatic cell transfer (which is, in effect, cloning). The standard ... full-grown pig can then be used to produce other transgenic pigs or the organs can be removed for human use. Oh, and by the way, the human immune ...

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by biohazard
Thu Jul 23, 2009 12:17 pm
 
Forum: Genetics
Topic: Help with Xenotransplantation please.
Replies: 1
Views: 4490

pH of medium for lymphocyte

... that. Since thymus contains nearly homogenous cells, I want to use that organ. Yes, here PBS is used to wash the cells, and as a temporary medium ... for some minutes before applying the dye (trypan blue). I think the culture medium is not necessary in this simple experiment. Or?

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by DoctorStein
Thu Jun 26, 2008 7:12 am
 
Forum: Molecular Biology
Topic: pH of medium for lymphocyte
Replies: 9
Views: 3868

How can I isolate adult stem cells from circulation.

... undifferentiated cell found among differentiated cells in a tissue or organ, can renew itself, and can differentiate to yield the major specialized ... laboratories are trying to find ways to grow adult stem cells in cell culture and manipulate them to generate specific cell types so they can be ...

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by biolearner
Mon May 19, 2008 10:07 pm
 
Forum: Cell Biology
Topic: How can I isolate adult stem cells from circulation.
Replies: 1
Views: 6590

Re: question about skin cells elaboration

... People are trying, but you can’t do that sort of thing (yet) with an organ like the liver or kidney. Skin cells (well, specifically fibroblasts) ... division in vitro without the use of a lot of exotic additives to the culture media. In contrast, primary explants of liver or kidney cells sometimes ...

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by blcr11
Wed Apr 30, 2008 9:12 pm
 
Forum: Genetics
Topic: question about skin cells elaboration
Replies: 1
Views: 1991

Sugar Allergy

... 34. Kruzel ML, Harari Y, Chen C-Y, et al. The gut: a key metabolic organ protected by lactoferrin during experimental systemic inflammation ... acidophilus toward intestinal and food borne pathogens in associative cultures. J Food Protection 1977;40(12):820-23. 61. Schauss AG. Lactobacillus ...

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by DrDahlman
Sat Jan 12, 2008 4:20 pm
 
Forum: Human Biology
Topic: Sugar Allergy
Replies: 76
Views: 229994
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