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Orf

orf

A specific disease of sheep and goats, caused by the orf virus. This virus is transmissible to man and characterised by vesiculation and ulceration of the infected site.

Synonym: contagious ecthyma, contagious pustular dermatitis, scabby mouth, soremouth.

Origin: O.E. Orfcwealm, murrain, fr. Orf, cattle, _ cwealm, destruction


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Re: Virology - Calculations

**A virus has a genome of 1.184 MB (1184 kb) of double stranded DNA. 90.5% is coded into 1262 ORFs. What would be the average size of a coded protein in kiloDaltons? -1184 kb is 1184000 bases times the 660 Da per base pair gives 7.8144x10^8Daltons... Not sure where to ...

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by JackBean
Tue Sep 17, 2013 3:58 pm
 
Forum: General Discussion
Topic: Virology - Calculations
Replies: 1
Views: 1514

need help with subcloning !!

... i am a bit lost!! clone 1 : the desired fragment is present with the RE sites but there is a stop codon just after the flag ha tag in the correct ORF. I dont get it ?? clone2 and 3: the desired fragment is present in the correct ORF with the n-terminal flag ha tag but I cant find the site for ...

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by Jasmine0507
Wed Dec 21, 2011 1:20 pm
 
Forum: Molecular Biology
Topic: need help with subcloning !!
Replies: 3
Views: 1827

Superhuman abilities

ankit: but the rest of genome is not coding, because it has no ORF, not that it wouldn't be transcribed. Au contraire, most of our genome is actually transcribed and as we are learning just recently, it has probably regulatory role.

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by JackBean
Fri Sep 23, 2011 1:31 pm
 
Forum: General Discussion
Topic: Superhuman abilities
Replies: 36
Views: 18449

Cloning with pBR322 and two restriction enzymes

... into plasmid you need to find enzymes that cut once. Imagine you take an enzyme that cuts near the cloning site but also in the middle of an orf (e.g. antibiotic resistance gene). Then you would lose a big but important part of the plasmid. That means to have more site the enzymes cut you ...

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by Papaver
Tue Aug 16, 2011 1:05 pm
 
Forum: Molecular Biology
Topic: Cloning with pBR322 and two restriction enzymes
Replies: 4
Views: 4297

PCR with one lone primer and another short

... 30mer forward primer. I need to fuse His and V5 Tag to my protein, so i have designed those tagged sequences in my reverse primer to amplify the ORF with tagged seqence and later clone it into a vector. Can anyone please suggest how to proceed with such primer or whether my approach is wrong... ...

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by rama12chandra
Mon Jul 18, 2011 3:09 pm
 
Forum: Molecular Biology
Topic: PCR with one lone primer and another short
Replies: 2
Views: 1175
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