Optimal

There must be some synchronization cues; otherwise many cells would end up with over- or under-optimal numbers of mitochondria. Since many of the critical proteins in a mitochondrian are nuclear-encoded, the proliferation of the mitochondria can be limited by the availability ...

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... to mice from the optimized concentration that elicits an effect in a cell-culture based system (nM)? For example, if I empirically determined the optimal concentration of reagent ___ for an effect in ___ cell line is 100nM, how do I calculate the dose to be given to mice from it? Converesly, if ...

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Hello everyone, I am having some difficulty determining the optimal Tm for my primers for PCR. I ordered two primers for different genes. gene A primers Tm 59 and 71 gene B primers Tm 64 and 71 I am trying to run them both at the same time since they amplify ...

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Your answer is difficult to read and confusing. Some things jump out. Instead of saying "annealing temperature of a PCR is too low for optimal primer function" you should say "annealing temperature below optimal". Also in the last sentence "reduce the quantity of product ...

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... of a PCR was too low for the primers in the reaction? Explain My Answer: In cases in which the annealing temperature of a PCR is too low for optimal primer function, annealing may occur at non-specific or mismatched sites compared to the primer’s true target. This occurs as at sufficient ...

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