Dictionary » O » Optical density

Optical density

Definition

noun

(1) The measure of transmittance of an optical medium for a given wavelength. The higher the OD the lower the transmittance, the higher protection factor by a filter (e.g. goggles, viewing windows, etc.).

(2) The measure of the amount of light absorbed by a suspension of bacterial cells or a solution of an organic molecule with the use of a colourimeter or spectrophotometer.


Supplement

In microbiology, the values can be used to measure turbidity, which in turn is used to estimate the number of bacteria (or concentration of molecules like proteins) in a solution.


Abbreviation: OD

Synonym: absorbance.
See also: absorption.


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


Chemical effects on bacteria

or measure the optical density,provided you keep a control without the substance

See entire post
by lara
Tue Jun 09, 2009 11:37 am
 
Forum: Microbiology
Topic: Chemical effects on bacteria
Replies: 4
Views: 206

Optical density Q

In an experiment it is found that the optical density of a solution of compound X was 0.7 when measured at 400nm. in a 1cm glass cuvette. The solution ...

See entire post
by monkey07
Mon Jun 01, 2009 4:34 pm
 
Forum: Molecular Biology
Topic: Optical density Q
Replies: 0
Views: 409

Re: Biochem Help?

... volume of stock solution needs to be made up to 10 ml? (4 marks) 7. The optical density OD (or absorbance) at 600 nm of an overnight culture of the bacterium Vibrio fischeri ...

See entire post
by canalon
Wed Nov 28, 2007 2:37 pm
 
Forum: Molecular Biology
Topic: Biochem Help?
Replies: 14
Views: 1480

Biochem Help?

... volume of stock solution needs to be made up to 10 ml? (4 marks) 7. The optical density OD (or absorbance) at 600 nm of an overnight culture of the bacterium Vibrio fischeri ...

See entire post
by Chemhalp
Wed Nov 28, 2007 2:24 pm
 
Forum: Molecular Biology
Topic: Biochem Help?
Replies: 14
Views: 1480

Combining extinction coefficients?

... method work or am I way off? A solution containing NAD+ and NADH has an optical density in a 1 cm cuvette of 0.311 at 340 nm and of 1.2 at 260 nm. Calculate the concentrations ...

See entire post
by dae
Tue Sep 18, 2007 9:25 pm
 
Forum: Molecular Biology
Topic: Combining extinction coefficients?
Replies: 4
Views: 1881
View all matching forum results

This page was last modified 10:25, 24 July 2008. This page has been accessed 16,824 times. 
What links here | Related changes | Permanent link