Oocyte

My lysis buffer: NP40 2ul RNase Inhibitor 5ul dNTP 10ul oligodT 10ul DTT 10ul 5*RT buffer 40ul Add H2O to 100ul， Take 5ul to eppendorf tube. hyaluronidase to remove cumulus cells. The amplification program for the reverse transcription step was as follows: 70°C for 10 min, ice 2min ,MMLV ,42°C for 5...

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... it. It it's not fertilized within 10-14 days then the uterus will in turn no longer need progesterone and estrogen to ready for pregnancy. If the oocyte is fertilized and does becomes a zygote then the corpus luteum will continue to secrete progesterone and estrogen for 12 weeks in order soften ...

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... been already made) Menstral cycle: Day 1-14 = LH production increases slowly (effect of FSH production cauising development of thecal cells of oocyte) Day 14 ('ovulation') = LH surge (positive feedback due to oestrogen) Day 14-28 = High progesterone -> Inhibition of LH [NB. Day 28 is menstration] ...

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... same explanation, i was just doubtful whether RBC can survive just with the proteins made before the maturation) what i meant in the case of the oocyte was, that during division the transcribtion is halted and since the primary oocyte stops at the middle of it (diplotene stage) proteine production ...

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I can't think of a reason why a primary oocyte won't be able to undergo transciption...

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