Dictionary » O » Oligosaccharides

Oligosaccharides

oligosaccharide

(Science: chemistry) A saccharide of a small number of component sugars, either O or N linked to the next sugar. Number of component sugars not rigorously defined - the range of 3 to 10 component sugars is commony accepted.


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Re: N-linked oligosaccharides

1) Is the oligosaccharide underivatised, permethylated or peracetylated? 2) Would you like me to tell you the mass values as monoisotopic or average? SO: 3) Tell me what oligosaccharide and I'll guide you through it.

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by billyfisher100
Sat Jan 21, 2012 1:34 pm
 
Forum: General Discussion
Topic: N-linked oligosaccharides
Replies: 1
Views: 760

N-linked oligosaccharides

Does anyone have any idea how you estimate the average mass of an n-linked oligosaccharide? Thanks in anticipation,

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by maegovannen
Thu Jan 05, 2012 11:51 pm
 
Forum: General Discussion
Topic: N-linked oligosaccharides
Replies: 1
Views: 760

SDS-PAGE analysis

... stupid.. how do I estimate the NLOmass? I'm confused as to how I can use this to calculate the NLOmass, as there are so many different n-linked oligosaccharides? Thank you in advance,

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by madinacath
Thu Jan 05, 2012 11:27 pm
 
Forum: Cell Biology
Topic: SDS-PAGE analysis
Replies: 4
Views: 2444

Re: SDS-PAGE analysis

Estimate the average mass of an n-linked oligosaccharides. Let that mass = NLOmass (g/mole) Subtract the mass of the protein after PNGase F treatment from the mass of the untreated protein. Let that mass = DiffMass (g/mole). DiffMass/NLOmass ...

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by jonmoulton
Thu Jan 05, 2012 11:11 pm
 
Forum: Cell Biology
Topic: SDS-PAGE analysis
Replies: 4
Views: 2444

SDS-PAGE analysis

I need to find the number of n-linked oligosaccharides in different secreted proteins. I've completed an SDS-PAGE experiment and have a set of results. I've drawn a calibration curve of the molecular marker and therefore have an estimate of ...

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by madinacath
Thu Jan 05, 2012 10:16 pm
 
Forum: Cell Biology
Topic: SDS-PAGE analysis
Replies: 4
Views: 2444
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