Oligosaccharides

1) Is the oligosaccharide underivatised, permethylated or peracetylated? 2) Would you like me to tell you the mass values as monoisotopic or average? SO: 3) Tell me what oligosaccharide and I'll guide you through it.

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Does anyone have any idea how you estimate the average mass of an n-linked oligosaccharide? Thanks in anticipation,

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... stupid.. how do I estimate the NLOmass? I'm confused as to how I can use this to calculate the NLOmass, as there are so many different n-linked oligosaccharides? Thank you in advance,

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Estimate the average mass of an n-linked oligosaccharides. Let that mass = NLOmass (g/mole) Subtract the mass of the protein after PNGase F treatment from the mass of the untreated protein. Let that mass = DiffMass (g/mole). DiffMass/NLOmass ...

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I need to find the number of n-linked oligosaccharides in different secreted proteins. I've completed an SDS-PAGE experiment and have a set of results. I've drawn a calibration curve of the molecular marker and therefore have an estimate of ...

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