Dictionary » O » Oligonucleotides

Oligonucleotides

oligonucleotides

polymers made up of a few (2-20) nucleotides. In molecular genetics, they refer to a short sequence synthesised to match a region where a mutation is known to occur, and then used as a probe (oligonucleotide probes).


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


Re: Any ideas for vaccine development or anti-viral drugs???

... triphosphatase activity reduced budding by up to 80%. Inhibition of VPS4 gene expression by use of phosphorodiamidite morpholino antisense oligonucleotides protected mice from lethal EBOV infection. These data show that EBOV can use vps proteins independently of TSG101 for budding and reveal ...

See entire post
by jonmoulton
Mon Sep 15, 2014 3:04 pm
 
Forum: Cell Biology
Topic: Any ideas for vaccine development or anti-viral drugs???
Replies: 2
Views: 479

TINA and TFO

can you please help me to understand the relationship betewwn Twisted Intercalating Nucleic Acid & Triples Oligonucleotides ???

See entire post
by Helics
Fri Oct 19, 2012 2:38 pm
 
Forum: Molecular Biology
Topic: TINA and TFO
Replies: 0
Views: 717

Re: question for evolutionary biologist.

... was designed based on Genbank Accession Number CP001668 (Mycoplasma mycoides subs. capri str. GM12), was assembled from chemically synthesized oligonucleotides. The sequenced genome was isolated from a yeast clone. The sequenced clone is sMmYCp235-1. This synthetic genome contains all but 14 ...

See entire post
by scottie
Thu Sep 13, 2012 1:22 pm
 
Forum: Evolution
Topic: question for evolutionary biologist.
Replies: 26
Views: 28516

How to prove that thiol groups are de-protected?

... where thiol group reacts with C=C electron deficient bond. Befor trying to work with proteins, which could be hard, I'm trying to ligate two DNA oligonucleotides, one modified with thiol group, another is modified with acrydite group: DNA1--SH + CH2=CH--C(O)--DNA2 ---> DNA1--S--CH(CH3)--C(O)--DNA2 ...

See entire post
by sergii
Wed May 02, 2012 7:32 pm
 
Forum: Molecular Biology
Topic: How to prove that thiol groups are de-protected?
Replies: 2
Views: 1505

Designing primers to clone into expression vector

... on the site directed mutagenesis wiki page it says "Variations employ three or four oligionucleotides, two of which may be non-mutagenic oligonucleotides that cover two convenient restriction sites and generate a fragment that can be digested and ligated into a plasmid, while the mutagenic ...

See entire post
by Jemi
Wed Feb 15, 2012 8:11 pm
 
Forum: Molecular Biology
Topic: Designing primers to clone into expression vector
Replies: 1
Views: 1804
View all matching forum results

This page was last modified 21:16, 3 October 2005. This page has been accessed 11,327 times. 
What links here | Related changes | Permanent link