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Oligonucleotides

oligonucleotides

polymers made up of a few (2-20) nucleotides. In molecular genetics, they refer to a short sequence synthesised to match a region where a mutation is known to occur, and then used as a probe (oligonucleotide probes).


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Taqman probe design using FISH probe (need help)

... DNA (metaphase chromosomes or interphase nuclei). Hydrolysis probes (such as Taqman) are very short and used in combination with other two oligonucleotides. the size of the amplicon is very small, usually < 200 bp. here, the hydrolysis probe has an annealing temperature such that it ...

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by protoemics
Wed Feb 04, 2015 8:11 am
 
Forum: Bioinformatics
Topic: Taqman probe design using FISH probe (need help)
Replies: 2
Views: 543

Re: Any ideas for vaccine development or anti-viral drugs???

... triphosphatase activity reduced budding by up to 80%. Inhibition of VPS4 gene expression by use of phosphorodiamidite morpholino antisense oligonucleotides protected mice from lethal EBOV infection. These data show that EBOV can use vps proteins independently of TSG101 for budding and reveal ...

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by jonmoulton
Mon Sep 15, 2014 3:04 pm
 
Forum: Cell Biology
Topic: Any ideas for vaccine development or anti-viral drugs???
Replies: 2
Views: 12575

TINA and TFO

can you please help me to understand the relationship betewwn Twisted Intercalating Nucleic Acid & Triples Oligonucleotides ???

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by Helics
Fri Oct 19, 2012 2:38 pm
 
Forum: Molecular Biology
Topic: TINA and TFO
Replies: 0
Views: 838

Re: question for evolutionary biologist.

... was designed based on Genbank Accession Number CP001668 (Mycoplasma mycoides subs. capri str. GM12), was assembled from chemically synthesized oligonucleotides. The sequenced genome was isolated from a yeast clone. The sequenced clone is sMmYCp235-1. This synthetic genome contains all but 14 ...

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by scottie
Thu Sep 13, 2012 1:22 pm
 
Forum: Evolution
Topic: question for evolutionary biologist.
Replies: 26
Views: 34559

How to prove that thiol groups are de-protected?

... where thiol group reacts with C=C electron deficient bond. Befor trying to work with proteins, which could be hard, I'm trying to ligate two DNA oligonucleotides, one modified with thiol group, another is modified with acrydite group: DNA1--SH + CH2=CH--C(O)--DNA2 ---> DNA1--S--CH(CH3)--C(O)--DNA2 ...

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by sergii
Wed May 02, 2012 7:32 pm
 
Forum: Molecular Biology
Topic: How to prove that thiol groups are de-protected?
Replies: 2
Views: 1807
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