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Oligonucleotides

oligonucleotides

polymers made up of a few (2-20) nucleotides. In molecular genetics, they refer to a short sequence synthesised to match a region where a mutation is known to occur, and then used as a probe (oligonucleotide probes).


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TINA and TFO

can you please help me to understand the relationship betewwn Twisted Intercalating Nucleic Acid & Triples Oligonucleotides ???

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by Helics
Fri Oct 19, 2012 2:38 pm
 
Forum: Molecular Biology
Topic: TINA and TFO
Replies: 0
Views: 567

Re: question for evolutionary biologist.

... was designed based on Genbank Accession Number CP001668 (Mycoplasma mycoides subs. capri str. GM12), was assembled from chemically synthesized oligonucleotides. The sequenced genome was isolated from a yeast clone. The sequenced clone is sMmYCp235-1. This synthetic genome contains all but 14 ...

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by scottie
Thu Sep 13, 2012 1:22 pm
 
Forum: Evolution
Topic: question for evolutionary biologist.
Replies: 26
Views: 21850

How to prove that thiol groups are de-protected?

... where thiol group reacts with C=C electron deficient bond. Befor trying to work with proteins, which could be hard, I'm trying to ligate two DNA oligonucleotides, one modified with thiol group, another is modified with acrydite group: DNA1--SH + CH2=CH--C(O)--DNA2 ---> DNA1--S--CH(CH3)--C(O)--DNA2 ...

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by sergii
Wed May 02, 2012 7:32 pm
 
Forum: Molecular Biology
Topic: How to prove that thiol groups are de-protected?
Replies: 2
Views: 1166

Designing primers to clone into expression vector

... on the site directed mutagenesis wiki page it says "Variations employ three or four oligionucleotides, two of which may be non-mutagenic oligonucleotides that cover two convenient restriction sites and generate a fragment that can be digested and ligated into a plasmid, while the mutagenic ...

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by Jemi
Wed Feb 15, 2012 8:11 pm
 
Forum: Molecular Biology
Topic: Designing primers to clone into expression vector
Replies: 1
Views: 1464

Re: Theories - Origin of Life

... the assembly of nucleosides from component sugars and nucleobases, the assembly of nucleotides from nucleosides and phosphate, and the assembly of oligonucleotides from nucleotides are all thermodynamically uphill in water. That is also true for polypeptide chains that join amino acids. Two amino ...

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by scottie
Fri Aug 19, 2011 12:31 pm
 
Forum: Evolution
Topic: Theories - Origin of Life
Replies: 559
Views: 755238
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