Oligonucleotides

can you please help me to understand the relationship betewwn Twisted Intercalating Nucleic Acid & Triples Oligonucleotides ???

See entire post

... was designed based on Genbank Accession Number CP001668 (Mycoplasma mycoides subs. capri str. GM12), was assembled from chemically synthesized oligonucleotides. The sequenced genome was isolated from a yeast clone. The sequenced clone is sMmYCp235-1. This synthetic genome contains all but 14 ...

See entire post

... where thiol group reacts with C=C electron deficient bond. Befor trying to work with proteins, which could be hard, I'm trying to ligate two DNA oligonucleotides, one modified with thiol group, another is modified with acrydite group: DNA1--SH + CH2=CH--C(O)--DNA2 ---> DNA1--S--CH(CH3)--C(O)--DNA2 ...

See entire post

... on the site directed mutagenesis wiki page it says "Variations employ three or four oligionucleotides, two of which may be non-mutagenic oligonucleotides that cover two convenient restriction sites and generate a fragment that can be digested and ligated into a plasmid, while the mutagenic ...

See entire post

... the assembly of nucleosides from component sugars and nucleobases, the assembly of nucleotides from nucleosides and phosphate, and the assembly of oligonucleotides from nucleotides are all thermodynamically uphill in water. That is also true for polypeptide chains that join amino acids. Two amino ...

See entire post