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Oligonucleotides

oligonucleotides

polymers made up of a few (2-20) nucleotides. In molecular genetics, they refer to a short sequence synthesised to match a region where a mutation is known to occur, and then used as a probe (oligonucleotide probes).


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Re: Theories - Origin of Life

... the assembly of nucleosides from component sugars and nucleobases, the assembly of nucleotides from nucleosides and phosphate, and the assembly of oligonucleotides from nucleotides are all thermodynamically uphill in water. That is also true for polypeptide chains that join amino acids. Two amino ...

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by scottie
Fri Aug 19, 2011 12:31 pm
 
Forum: Evolution
Topic: Theories - Origin of Life
Replies: 265
Views: 28703

Re: Theories - Origin of Life

... designed cassettes were generally 1,080 bp with 80-bp overlaps to adjacent cassettes. They were all produced by assembly of chemically synthesized oligonucleotides by Blue Heron; Bothell, Washington. Each cassette was individually synthesized and sequence-verified by the manufacturer. They made ...

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by scottie
Mon May 30, 2011 3:28 pm
 
Forum: Evolution
Topic: Theories - Origin of Life
Replies: 265
Views: 28703

cloning

Hola, Today to clone big genes the way is amplify them with PCR from the whole DNA of the organism which carry it. First we design two oligonucleotides wich hybridize with the 5´and 3´ends and carry restriction endonucleases sites to introduce the gene in a subcloning plasmid and after production ...

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by protold
Thu May 05, 2011 5:54 am
 
Forum: Molecular Biology
Topic: cloning
Replies: 10
Views: 1093

Re:

... for saccharides, the break point would be 10 units. For other molecules it might be longer (DNA primers, typically 20 to 30 nt long will still be oligonucleotides) oligo means 10 and ploy means more then ten according to me

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by vinayaksabnis
Fri Oct 08, 2010 7:29 am
 
Forum: Molecular Biology
Topic: Diff b/w: monosaccharide, oligosaccharide, polysaccharide?
Replies: 7
Views: 1724

Re:

... for saccharides, the break point would be 10 units. For other molecules it might be longer (DNA primers, typically 20 to 30 nt long will still be oligonucleotides) Okay so polys are made out of a LOT of monos?

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by leftventricle
Thu Oct 07, 2010 11:28 pm
 
Forum: Molecular Biology
Topic: Diff b/w: monosaccharide, oligosaccharide, polysaccharide?
Replies: 7
Views: 1724
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