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Oligonucleotide

oligonucleotide

(Science: biochemistry) linear sequence of up to 20 nucleotides joined by phosphodiester bonds. Above this length the term polynucleotide begins to be used. A short sequence of [[nucleotides.


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Data analysis: DNA alignment HELP?

... a CDK in maize, investigators aligned the predicted amino acid sequences of human cdc2, S. pombe cdc2, and S. cerevisiae cdc28 genes. Degenerate oligonucleotide PCR primers corresponding to the boxed amino acids were generated and a DNA fragment of the expected size was synthesized by PCR using ...

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by TToe
Mon Feb 18, 2013 10:40 am
 
Forum: Genetics
Topic: Data analysis: DNA alignment HELP?
Replies: 1
Views: 1785

Do the simplest bacteria have ribosomes and helicase?

... and exactly one phospate group in-between. For synthesis of RNA ATP, CTP, GTP and UTP are used. As such, there is ATP among building blocks of an oligonucleotide. ATP, AMP, ADP and AAUUC are all RNAs, although first three more commonly called nucleotides, while RNA is generally refered to oligos. ...

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by dustman
Sat May 26, 2012 11:04 pm
 
Forum: Molecular Biology
Topic: Do the simplest bacteria have ribosomes and helicase?
Replies: 27
Views: 13357

How to prove that thiol groups are de-protected?

Hi everyone. I'm trying to link chemically a protein with a DNA oligonucleotide. I think one of the simplest way would be to use Michael addition, where thiol group reacts with C=C electron deficient bond. Befor trying to work with proteins, which could ...

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by sergii
Wed May 02, 2012 7:32 pm
 
Forum: Molecular Biology
Topic: How to prove that thiol groups are de-protected?
Replies: 2
Views: 1393

Designing primers to clone into expression vector

... on the site directed mutagenesis wiki page it says "Variations employ three or four oligionucleotides, two of which may be non-mutagenic oligonucleotides that cover two convenient restriction sites and generate a fragment that can be digested and ligated into a plasmid, while the mutagenic ...

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by Jemi
Wed Feb 15, 2012 8:11 pm
 
Forum: Molecular Biology
Topic: Designing primers to clone into expression vector
Replies: 1
Views: 1710

Re:

... amniocentesis sampling could be analyzed by restriction enzyme digestion, electrophoresis, southern transfer and hybridization to a cloned gene or oligonucleotide probes (3). However, southern blotting permitted only rudimentary mapping of genes in unrelated individuals (4). It is generally considered ...

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by canalon
Sat May 14, 2011 3:02 am
 
Forum: Molecular Biology
Topic: History of Polymerase Chain Reaction
Replies: 5
Views: 2942
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