Oligonucleotide

... a CDK in maize, investigators aligned the predicted amino acid sequences of human cdc2, S. pombe cdc2, and S. cerevisiae cdc28 genes. Degenerate oligonucleotide PCR primers corresponding to the boxed amino acids were generated and a DNA fragment of the expected size was synthesized by PCR using ...

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... and exactly one phospate group in-between. For synthesis of RNA ATP, CTP, GTP and UTP are used. As such, there is ATP among building blocks of an oligonucleotide. ATP, AMP, ADP and AAUUC are all RNAs, although first three more commonly called nucleotides, while RNA is generally refered to oligos. ...

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Hi everyone. I'm trying to link chemically a protein with a DNA oligonucleotide. I think one of the simplest way would be to use Michael addition, where thiol group reacts with C=C electron deficient bond. Befor trying to work with proteins, which could ...

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... on the site directed mutagenesis wiki page it says "Variations employ three or four oligionucleotides, two of which may be non-mutagenic oligonucleotides that cover two convenient restriction sites and generate a fragment that can be digested and ligated into a plasmid, while the mutagenic ...

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... amniocentesis sampling could be analyzed by restriction enzyme digestion, electrophoresis, southern transfer and hybridization to a cloned gene or oligonucleotide probes (3). However, southern blotting permitted only rudimentary mapping of genes in unrelated individuals (4). It is generally considered ...

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