Oligonucleotide

... Sep 8. [Epub ahead of print] Yin H, Moulton HM, Seow Y, Boyd C, Boutilier J, Iverson P, Wood MJ. Cell-penetrating peptide-conjugated antisense oligonucleotides restore systemic muscle and cardiac dystrophin expression and function. Hum Mol Genet. 2008 Sep 10. [Epub ahead of print] Wu B, Moulton ...

See entire post

... exonic splicing enhancer motif that results in afibrinogenemia caused by aberrant mRNA splicing, which can be corrected in vitro with antisense oligonucleotide treatment. Hum Mutat. 2008 Oct 13. [Epub ahead of print] http://www.ncbi.nlm.nih.gov/pubmed/18853456

See entire post

Hello everyone, I have a question about Oligo-N directed mutagensis. after the primer is set and the DNA(plasmid) is replicated, this Hetroduplex is moved into a bacterium (Ecoli). theoretically when the cells replicate each DNA piece (mutated DNA & WildType DNA) will have equal chance of segreg...

See entire post

Depending on which table you want to use, NdeI requires 7 extra bases to cut a small oligonucleotide, or at least 6 extra bases to be able to efficiently cut an NdeI site near the end of a linear fragment. XmaI isn't nearly so sensitive to being near an end--it only ...

See entire post

... with a simple phenol extraction/ethanol precipitation step instead since the smaller nucleiotides don't precipitate as efficiently as the larger oligonucleotide, but usually you do a gel purification to be safe. You also digest a few micrograms of pET30 with NdeI/XhoI and purify the digested ...

See entire post