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Oil bath

oil bath

In chemistry, a vessel containing oil, in which a container holding a substance to be heated or evaporated can be immersed.


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Re: 2.5 M glycine crystallized

... warming the bottle and see if the crystals go back into solution. Leaving it on the countertop overnight might be enough. If not, a 37C water bath might work. Autoclaving is very likely to do the job -- be sure to use the liquid cycle. Solvent collisions can shake the molecules off the crystals, ...

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by jonmoulton
Fri Jun 20, 2014 2:45 pm
 
Forum: Molecular Biology
Topic: 2.5 M glycine crystallized
Replies: 1
Views: 887

Restriction Digesion

Both of them are okay! But i would go for Thermo-cycler instead of water bath.

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by MOmarKhan
Mon Apr 07, 2014 2:09 am
 
Forum: Molecular Biology
Topic: Restriction Digesion
Replies: 3
Views: 943

Restriction Digesion

which is the best option for Restriction Endonuclease digesion ??? A) 37' C water bath B) 37' C PCR program

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by Helics
Sun Apr 06, 2014 4:07 pm
 
Forum: Molecular Biology
Topic: Restriction Digesion
Replies: 3
Views: 943

Re: sterilization in microwave oven

... know of the older ways of sterilization when autoclaves were not available or too expensive for the purpose. Nurses used to use a boiling water bath to 'sterilize' needles for reuse and to 'sterilize' surgical instruments. It is innocence pure and simple with all authority for sterile in the ...

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by fdgsr
Tue Mar 18, 2014 3:13 pm
 
Forum: Microbiology
Topic: sterilization in microwave oven
Replies: 29
Views: 103001

Problems making chemically competent cells

... 0.4-0.6 (4-7x107 cells/ml). 4. When at the correct OD, chill the culture on ice for 10-15 minutes. I use a pre-chilled flask for this in a wet ice bath. 5. Spin the culture at 3000 RPM for 15 minutes at 4°C in a pre-chilled rotor. 6. Resuspend the pellet in RF1 solution. The volume should be 1/3 ...

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by lilruthc
Fri Feb 08, 2013 1:09 pm
 
Forum: Molecular Biology
Topic: Problems making chemically competent cells
Replies: 1
Views: 2670
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