O-antigen polymerase

I agree with Jack. Polymerase adds bases by elongating from the 3' end of a nucleic acid strand which is bound in an antiparallel orientation with the template strand. I have not heard of priming the template backwards (parallel). ...

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... so far used varying primers on the cDNA of arabidopsis in order to get some fragments that I could then ligate together. I have used proofreading polymerase, Taq, phusion, abgene, and I have then tried (and failed) to isolate, purify and sequence the fragment. The sequence results I get are always ...

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Ques1: Phosphorylation of RNA pol which are the roles of the phosphorylaton of C terminal Domain of RNA polymerase? (more than one may apply) 1. It increases the speed of transcription 2. It increase the specificity of the transcription process 3. It pauses transcription to ...

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... about when one job involves two enzymes ie. DNA Gyrase and DNA Helicase in unwinding the double helix or when an enzyme has two jobs such as DNA Polymerase forming phosphodiester bonds between nucleotides and adding new nucleotides to the RNA Primer. Also, what is Proeolytic Cleavage?

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I agree with what JackBean said, however it does not answer your question. MgCl2 is a required cofactor for the Taq Polymerase to work. The activity of the Taq polymerase is sensitive to the concentration of MgCl2. The higher the concentration of the MgCl2, the more active the enzyme ...

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