Dictionary » N » Nucleotide Sequence

Nucleotide Sequence

The order in which [[ nucleotides are situated in a chain relative to one another, which in future will provide the template of a particular [[amino acid, therefore making the order of the nucleotide sequence important.


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Pyrosequencing

Hello, I would like to sequence a gene region with a couple of mutation hot spots nearby each other, and I have ... hot spot, I add two dispensations - one for the wild type and one for the mutant nucleotide. Question1: how come that these hot spots always change from e.g. G to C and ...

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by kk
Wed Jan 23, 2013 3:08 pm
 
Forum: Molecular Biology
Topic: Pyrosequencing
Replies: 2
Views: 389

Re: Muscular dystrophy inheritance question

The lower sequence shows one trace for the male but for the female there are two traces at one nucleotide position (labelled N). That looks like the allelic difference; since the female has two copies of the ...

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by firefly
Wed Jan 09, 2013 9:16 pm
 
Forum: Molecular Biology
Topic: Muscular dystrophy inheritance question
Replies: 7
Views: 1071

Re: Muscular dystrophy inheritance question

The lower sequence shows one trace for the male but for the female there are two traces at one nucleotide position (labelled N). That looks like the allelic difference; since the female has two copies of the ...

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by jonmoulton
Wed Jan 09, 2013 5:06 pm
 
Forum: Molecular Biology
Topic: Muscular dystrophy inheritance question
Replies: 7
Views: 1071

Muscular dystrophy inheritance question

... dystrophy showing autosomal recessive inheritance. Genomic DNA was sequenced and the two mutations are shown for the affected. The corresponding ... gel of the same height and the computer wont be able to tell what the nucleotide is and that nucleotide is then assigned as N". How can it ...

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by firefly
Mon Jan 07, 2013 8:35 pm
 
Forum: Molecular Biology
Topic: Muscular dystrophy inheritance question
Replies: 7
Views: 1071

Nucleotide Database

PubMed Contains only about 500 nucleotide sequences related to Hibiscus http://www.ncbi.nlm.nih.gov/nuccore?term=%22Hibiscus%22[Organism]&cmd=DetailsSearch ...

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by JackBean
Tue Sep 25, 2012 9:19 am
 
Forum: Molecular Biology
Topic: Nucleotide Database
Replies: 9
Views: 1214
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