Dictionary » N » Nucleosome

Nucleosome

a DNA- histone complex, about 11nm in diameter; each nucleosome contains a 146- nucleotide- long streach of DNA and eight histine molecules (two each of H2A, H2B, H3, and H4).


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DNA

... they are integer multiples of the mass of the smallest rung -- that is, the masses are periodic. I've not done this. Anyone have experience with nucleosome periodicity?

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by jonmoulton
Thu Feb 14, 2013 4:32 pm
 
Forum: Genetics
Topic: DNA
Replies: 8
Views: 6329

DNA

Can you help me understand what exactly is nucleosome periodicity? We are studying it by adding micrococcal nuclease within the nucleus and studying it by SDS-PAGE.The results we had, showed several bands around 6 (nucleosomes that were cut by ...

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by simina
Thu Feb 14, 2013 1:41 pm
 
Forum: Genetics
Topic: DNA
Replies: 8
Views: 6329

Re:

... in the middle of the gel ending with strong band of low molecular weight)= 1. In this lane the DNA has been almost completely digested because the nucleosome had been extracted before nuclease treatment Lane 1 (smear starting at the well and ending in the middle of the gel means undigested DNA)= ...

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by 2468
Sat Nov 03, 2012 4:32 pm
 
Forum: Molecular Biology
Topic: Chromatin experiment
Replies: 10
Views: 3317

Chromatin experiment

... in the middle of the gel ending with strong band of low molecular weight)= 1. In this lane the DNA has been almost completely digested because the nucleosome had been extracted before nuclease treatment Lane 1 (smear starting at the well and ending in the middle of the gel means undigested DNA)= ...

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by Cat
Sat Nov 03, 2012 3:46 pm
 
Forum: Molecular Biology
Topic: Chromatin experiment
Replies: 10
Views: 3317

Chromatin experiment

... explanation for the bands visible in the different lanes. Explanations: 1. In this lane the DNA has been almost completely digested because the nucleosome had been extracted before nuclease treatment 2. The high molecular weight bands in this lane represent DNA protected from the nuclease 3. ...

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by 2468
Thu Nov 01, 2012 12:57 pm
 
Forum: Molecular Biology
Topic: Chromatin experiment
Replies: 10
Views: 3317
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