Nuclease

... your cDNA, red is radioactively labeled chromosomal DNA containing the gene for Beta globin. You first hybridize them. then treat with either S1 nuclease or exonuclease VII. S1 nuclease is an enzyme that degrades all single stranded DNA, exonuclease VII only degrades single stranded DNA that ...

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... boiling and high salt - allowing the arms to "snap-back" on each other with rapid cooling - digesting away single stranded DNA with S1 nuclease but I'm still not getting the enrichment I want, or maybe too much single stranded DNA contamination. Any ideas are greatly appreciated, thank ...

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... BMP activates a TGF{beta} Receptor Ser/Thr Kinase pathway leading to activation of Smad 1,5 which dimerizes with Smad 4 then translocates to the nuclease where it acts as a transcription factor.

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... to be a problem with your vector DNA. It can’t be the enzymes—the controls are fine, if I understand you. The enzymes are not contaminated with nuclease or such—you still recover the pcr product after digestion, and since the single-digest controls appeared to work, there is no reason to suspect ...

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... to be a problem with your vector DNA. It can’t be the enzymes—the controls are fine, if I understand you. The enzymes are not contaminated with nuclease or such—you still recover the pcr product after digestion, and since the single-digest controls appeared to work, there is no reason to suspect ...

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