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Nuclease

nuclease

(Science: enzyme) An enzyme capable of cleaving the phosphodiester bonds between nucleotide subunits of nucleic acids. General term for enzymes that catalyze the hydrolysis of nucleic acid by cleaving chains of nucleotides into smaller units.An [[ enzyme promoting the [[hydrolysis of [[nucleic acids.


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DNA

I think this is a case where winding the DNA onto histones protects it from the nuclease, so the DNA is cut only in regions between the histones. If the same length of DNA is wound around each histone and every DNA segment between histones gets cut, the fragments ...

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by jonmoulton
Thu Feb 14, 2013 4:32 pm
 
Forum: Genetics
Topic: DNA
Replies: 8
Views: 6132

DNA

Can you help me understand what exactly is nucleosome periodicity? We are studying it by adding micrococcal nuclease within the nucleus and studying it by SDS-PAGE.The results we had, showed several bands around 6 (nucleosomes that were cut by the nuclease). I do not really know ...

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by simina
Thu Feb 14, 2013 1:41 pm
 
Forum: Genetics
Topic: DNA
Replies: 8
Views: 6132

Chromatin experiment

... weight bands? 1. Increase the EDTA concentration 2. Expose the cells to the detergent for longer time 3. Increase the concentration of the nuclease 4. Decrease the concentration of the nuclease 5. Increase the incubation time with the nuclease. 6. Increase the number of cells used I've ...

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by 2468
Thu Nov 08, 2012 7:48 pm
 
Forum: Molecular Biology
Topic: Chromatin experiment
Replies: 10
Views: 3198

Re:

... strong band of low molecular weight)= 1. In this lane the DNA has been almost completely digested because the nucleosome had been extracted before nuclease treatment Lane 1 (smear starting at the well and ending in the middle of the gel means undigested DNA)= 4. High molecular weight bands in this ...

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by 2468
Sat Nov 03, 2012 4:32 pm
 
Forum: Molecular Biology
Topic: Chromatin experiment
Replies: 10
Views: 3198

Chromatin experiment

... strong band of low molecular weight)= 1. In this lane the DNA has been almost completely digested because the nucleosome had been extracted before nuclease treatment Lane 1 (smear starting at the well and ending in the middle of the gel means undigested DNA)= 4. High molecular weight bands in this ...

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by Cat
Sat Nov 03, 2012 3:46 pm
 
Forum: Molecular Biology
Topic: Chromatin experiment
Replies: 10
Views: 3198
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