Dictionary » N » Northern blot

Northern blot

Northern blot

(Science: molecular biology, procedure) An electroblotting method in which rNA is transferred to a filter and detected by hybridisation to (32)P labelled RNA or dNA.


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


Re: qPCR vs Northern Blot

Thank you both for answers. Firstly - aliquots for Northern blot and qPCR were the same (firstly I did NB and after a couple of weeks qPCR). @jonmoulton - you're ...

See entire post
by Nevthonien
Tue Nov 12, 2013 2:47 pm
 
Forum: Molecular Biology
Topic: qPCR vs Northern Blot
Replies: 4
Views: 2578

Re: qPCR vs Northern Blot

I see that I reversed the sense of the observations - the Northern signal was maintained, the PRC signal decreased. A splicing change could still produce that result, as could the RNA degradation Jack mentioned.

See entire post
by jonmoulton
Mon Nov 11, 2013 4:38 pm
 
Forum: Molecular Biology
Topic: qPCR vs Northern Blot
Replies: 4
Views: 2578

Re: qPCR vs Northern Blot

I assume you are assaying aliquots of the same RNA samples by the two methods. Does your Northern probe bind between the binding sites of your PCR primers? If not, it is conceivable that a change in splicing in the presence of the shRNA causes your Northern target to ...

See entire post
by jonmoulton
Thu Nov 07, 2013 10:12 pm
 
Forum: Molecular Biology
Topic: qPCR vs Northern Blot
Replies: 4
Views: 2578

qPCR vs Northern Blot

Hello everyone, I have a question regarding comparison of results from northern blot and qPCR. I have RNA isolated from samples in which my GOI should be silenced (samples ...

See entire post
by Nevthonien
Thu Nov 07, 2013 8:35 pm
 
Forum: Molecular Biology
Topic: qPCR vs Northern Blot
Replies: 4
Views: 2578

nuclear acid probe labeling

... is available was generally a very good information for all Southern and Northern blot related thing (even if you use other products)

See entire post
by canalon
Sat Sep 25, 2010 6:16 pm
 
Forum: Molecular Biology
Topic: nuclear acid probe labeling
Replies: 3
Views: 2571
View all matching forum results

This page was last modified 21:16, 3 October 2005. This page has been accessed 2,274 times. 
What links here | Related changes | Permanent link