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Non specific endonuclease

Non-specific endonucleases:

restriction enzymes that do not recognise a specific dna recognition sequence. An example is DnaseI for double stranded dna


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gel electrophoresis & assays help please

... pSP1 and purify from e.coli. 2. cut pHG327 and pSP1 with restriction endonuclease HindIII. 3. fragments of pSP1 added to cut pHG327 to allow recombination ... of alginate lyase by recombinants will be quantified by measurement of specific enzyme activity i am having 2 problems... I have 2 gels ...

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by RachBio
Fri Feb 06, 2009 11:32 pm
 
Forum: Molecular Biology
Topic: gel electrophoresis & assays help please
Replies: 6
Views: 7106

Amino acid sequence

yes, but by manipulation of specific regions? Ever heard of site-specific induced mutagenesis? It's kind of advanced ... mutate the DNA exactly at the site you want changed. Then you cut it up with an endonuclease and you insert it into your organism. Easy as 1-2-3. Actually not. Very ...

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by Jessy Springfield
Tue Oct 30, 2007 7:23 pm
 
Forum: Genetics
Topic: Amino acid sequence
Replies: 17
Views: 5659

Amino acid sequence

yes, but by manipulation of specific regions? Ever heard of site-specific induced mutagenesis? It's kind of advanced ... mutate the DNA exactly at the site you want changed. Then you cut it up with an endonuclease and you insert it into your organism. Easy as 1-2-3. Actually not. Very ...

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by MrMistery
Tue Oct 30, 2007 12:06 pm
 
Forum: Genetics
Topic: Amino acid sequence
Replies: 17
Views: 5659

The Fiber Disease

... plasmids are used as cloning vectors restriction enzyme. Restriction endonuclease: (Science: enzyme molecular biology) class of bacterial enzymes that cut dNA at specific sites. In bacteria their function is to destroy foreign DNA, such as that of bacteriophages ...

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by al
Sun Dec 24, 2006 4:08 am
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 4992215

DNA - Sticky and blunt ends- Explain

yeap, yeach endonuclease has a specific "clipping pattern". the enzyme will always cut the DNA in the same way, regardless of the environment ...

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by MrMistery
Fri Oct 20, 2006 6:14 pm
 
Forum: Genetics
Topic: DNA - Sticky and blunt ends- Explain
Replies: 6
Views: 16379
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